HEK293/SRE-Luc Stable Cell Line
The HEK293/SRE-Luc cell line is designed for measuring the potency of EGF or anti-EGFR, and monitoring MAPK/ERK signaling pathway activity.
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| M00927 | |
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| 询价 | |
| M00927 | |
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| Product Description | Recombinant HEK293 cells stably overexpress the luciferase reporter gene under the control of human serum response element (SRE). This stable cell line product is designed for measuring the In Vitro biological potency of EGF or EGFR antibodies. |
| Culture Properties | Adherent. |
| Stability | Stable through more than 15 passages with no significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL). |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | DMEM, 10% FBS, 300 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies) |
| Complete Growth Medium | DMEM (Cat. No. 10569-010, Life Technologies), 10% FBS |
| Freeze Medium-DATA | 90% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma) |
Figure 1. EGF-induced luciferase expression in HEK293/SRE-Luc cells. After stimulation by EGF (Z02691-100, Genscript), the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Varioskan LUX, Thermo Fisher). The RLU were plotted against the log of the cumulative doses of EGF (Mean ± SEM, n = 3). The EC50 of EGF on cells was 1.84 ng/ml.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RLU and starts at Bottom and goes to Top along a sigmoid curve.
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Figure 2. Functional evaluation of Cetuximab, the EGFR antibody, in HEK293/SRE-Luc cells. HEK293/SRE-Luc cells were dispensed into the microplate and incubated at 37℃ overnight prior to the addition of different concentrations of Cetuximab. After 30 minutes’ incubation, EGF was added into the plate and incubated at 37℃ for 6 hours. Luminescence signal was detected with the plate reader (Varioskan LUX, Thermo Fisher). »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
