| ￥4255 在线订购包邮(港澳台除外) | |
| L01167-1 | |
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| Product Description |
CH1CaptureXP Affinity Magnetic Beads are a novel affinity purification medium developed based on a highly specific CH1 binder. They are specifically designed for the selective capture and purification of antibodies and antibody fragments containing the human CH1 (Constant Heavy 1) domain. The product specifically binds to the CH1 domain of the antibody heavy chain through immobilized CH1 binder, enabling efficient capture of target antibodies or antibody fragments from cell culture supernatants, fermentation broths, and other complex biological samples. Unlike conventional Protein A-based purification, which primarily relies on recognition of the Fc region, CH1CaptureXP Affinity Magnetic Beads do not depend on an intact Fc structure and are therefore particularly suitable for the purification of bispecific antibodies, human Fab, F(ab')₂, partial antibody fragments, and other CH1-containing antibody formats. The product is suitable for the purification of Human IgG1, IgG2, and IgG4, meeting the purification needs of various antibody molecules and antibody fragments. Among these, Human IgG1 exhibits the highest binding capacity and strongest binding performance, enabling more efficient purification. The beads provide highly specific capture of proteins from a variety of expression systems, including E. coli, insect cells, and mammalian cells, with low background, high recovery, and compatibility with automated high-throughput workflows. These magnetic beads exhibit excellent binding selectivity, enabling efficient target protein capture and recovery while helping to minimize the co-purification of non-specific proteins, free light chains, and other impurities. This facilitates the recovery of highly purified target antibodies and antibody fragments. The product is suitable for laboratory-scale antibody purification, antibody fragment preparation, and downstream process development. |
| Key Features |
● Specifically recognizes the CH1 region of the antibody heavy chain, enabling highly selective capture while minimizing the co-purification of non-specific proteins, free light chains, and other impurities. ● Does not depend on an intact Fc region, making it an alternative capture medium for antibody molecules that are not suitable for Protein A-based purification. ● Exhibits excellent alkali stability and good reusability. |
| Ligand | immobilized CH1 binder |
| Binding Capacity | ≥35 mg hIgG/mL magnetic beads; ≥35 mg hIgG-Fab/mL magnetic beads |
| Matrix | Magnetic agarose beads |
| Average Particle Size | 37-100 μm |
| Elution Method | 0.1 M NaAc (pH 3.5) |
| Reuse Capability | ≥35 purification cycles when regenerated and stored as recommended. |
| Formulation | 50% settled beads suspended in PBS (pH 7.4) with 0.1% ProClin300 |
| Application |
Laboratory-scale antibody purification Antibody fragment preparation Downstream process development |
| Storage | Stored at 2°C~8°C. Do not store at 0°C or below 0°C to avoid freezing the beads. |
Binding capacity of CH1CaptureXP Affinity Magnetic Beads (GenScript, L01167) was evaluated using a panel of IgG and Fab molecules under identical purification conditions. »
Purity of a panel of IgG and Fab molecules purified using CH1CaptureXP Affinity Magnetic Beads (GenScript, L01167) was assessed by SDS-PAGE under non-reducing and reducing conditions. »
Binding capacity of CH1CaptureXP Affinity Magnetic Beads (GenScript, L01167) was evaluated after repeated regeneration with 0.1 M NaOH. The beads retained ≥80% of its initial binding capacity after 35 regeneration cycles. »
| Terms of Use |
Product is provided under a free, non-transferable, limited license to use the Product solely in the manner described in its accompanying Product documentation (including, without limitation, any instructions for use, user manuals, certificates of analysis (CoA), datasheets, labels, and other written or electronic materials supplied or referenced by GenScript) and solely for the stated purpose. Any other use may require a separate license from GenScript. This limited, non-transferable license is granted upon the purchase of the Product from GenScript or its authorized distributors. This provision shall not be construed as a warranty of merchantability or fitness for a particular purpose or commercial use. Any reproduction, distribution, modification, reverse engineering, cloning, reverse cloning, sequence determination, sublicensing, or use of the Product to develop competing products or services is prohibited without GenScript’s prior written consent. Third-Party Trademark Acknowledgement All product and company names mentioned in this manual are trademarks or registered trademarks of their respective owners, including but not limited to: Triton, a trademark of The Dow Chemical Company; and Tween, a trademark of Croda International Plc. These trademarks are used solely for the purpose of identifying compatible reagents, products, or technologies. GenScript is not sponsored by, affiliated with, or endorsed by the owners of these trademarks. |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
