| ￥11100 在线订购包邮(港澳台除外) | |
| L01166-1 | |
|
|
|
|
|
|
|
|
|
| 咨询产品问题 | |
| Product Description |
Anti-DYKDDDDK G2 Affinity Magnetic Beads are a high-performance protein affinity purification medium developed based on a highly specific FLAG® binder. They are specifically designed for the highly selective capture and purification of recombinant proteins containing a FLAG® tag. The product specifically binds to the FLAG® tag on the target protein through an immobilized FLAG® binder, enabling efficient capture of FLAG®-tagged proteins from cell culture supernatants, bacterial lysates, yeast, and other complex biological samples. Compared with the first-generation FLAG® affinity purification medium, this product offers a significantly higher binding capacity, allowing larger volumes or higher concentrations of samples to be processed with less magnetic beads. This improves purification efficiency while reducing magnetic bead consumption and the associated cost per sample. The magnetic beads exhibit excellent binding selectivity and high target protein recovery, effectively minimizing the co-purification of non-specific proteins and enabling the recovery of highly purified FLAG®-tagged proteins. The product is compatible with commonly used protein purification buffer systems and supports mild elution conditions, helping to preserve the structural integrity and biological activity of the target protein. Anti-DYKDDDDK G2 Affinity Magnetic Beads are suitable for recombinant protein purification, high-throughput protein screening, laboratory-scale protein preparation, and downstream purification process development. |
| Key Features |
● High binding capacity (10–25 mg/mL), 5–10 times higher than that of the first-generation FLAG® purification medium. ● High specificity with low non-specific background binding. ● Supports high-throughput and automated purification workflows. |
| Ligand | immobilized FLAG® binder |
| Binding Capacity | 10-25 mg protein/mL magnetic beads (actual binding capacity may vary depending on the target protein, tag position and molecular size) |
| Matrix | Magnetic agarose beads |
| Average Particle Size | 40-90 μm |
| Elution Method |
Acid Elution: 0.1 M Gly-HCl, pH 3.0 Alkaline Elution: 0.1 M Tris, pH 12.0 Peptide Competition Elution: 1 mg/mL DYKDDDDK peptide |
| Reuse Capability | 10 purification cycles when regenerated and stored as recommended. |
| Formulation | 50% settled beads suspended in Glycerol containing 0.1% ProClin 300 |
| Application |
Recombinant protein purification High-throughput protein screening Laboratory-scale protein preparation Downstream purification process development |
| Storage | Stored at -20°C. |
Binding capacity of Anti-DYKDDDDK G2 Affinity Magnetic Beads (GenScript, L01166) was compared with MonoRab™ Anti-DYKDDDDK Magnetic Beads (GenScript, L00835), using a diverse panel of DYKDDDDK-tagged proteins. Anti-DYKDDDDK G2 Affinity Magnetic Beads demonstrated substantially higher binding capacity than the previous-generation MonoRab™ Anti-DYKDDDDK Magnetic Beads across all proteins tested. »
Anti-DYKDDDDK G2 Affinity Magnetic Beads (GenScript, L01166) was evaluated using DYKDDDDK-tagged proteins with different molecular weights and tag positions. The purified proteins showed purity comparable to those obtained using previous-generation MonoRab™ Anti-DYKDDDDK Magnetic Beads (GenScript, L00835). »
Anti-DYKDDDDK G2 Affinity Magnetic Beads (GenScript, L01166) demonstrated excellent reusability and regeneration stability. Following regeneration with 2 M GuHCl for 15 min, the beads maintained 78% regeneration efficiency after 15 reuse cycles. »
| Terms of Use |
Product is provided under a free, non-transferable, limited license to use the Product solely in the manner described in its accompanying Product documentation (including, without limitation, any instructions for use, user manuals, certificates of analysis (CoA), datasheets, labels, and other written or electronic materials supplied or referenced by GenScript) and solely for the stated purpose. Any other use may require a separate license from GenScript. This limited, non-transferable license is granted upon the purchase of the Product from GenScript or its authorized distributors. This provision shall not be construed as a warranty of merchantability or fitness for a particular purpose or commercial use. Any reproduction, distribution, modification, reverse engineering, cloning, reverse cloning, sequence determination, sublicensing, or use of the Product to develop competing products or services is prohibited without GenScript’s prior written consent. Third-Party Trademark Acknowledgement All product and company names mentioned in this manual are trademarks or registered trademarks of their respective owners, including but not limited to: FLAG® , a trademark of Merck KGaA, Darmstadt, Germany; Triton, a trademark of The Dow Chemical Company; and Tween, a trademark of Croda International Plc. These trademarks are used solely for the purpose of identifying compatible reagents, products, or technologies. GenScript is not sponsored by, affiliated with, or endorsed by the owners of these trademarks. |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
