| ￥2780 在线订购包邮(港澳台除外) | |
| L01165-1 | |
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| Product description |
CH1CaptureXP Affinity Resin FF is a novel affinity chromatography resin developed with a highly specific CH1 binder, specifically designed for the selective capture and purification of antibodies and antibody fragments containing the human CH1 (Constant Heavy 1) domain. The resin utilizes an immobilized CH1-specific binder to selectively bind to the CH1 domain of the antibody heavy chain, enabling efficient capture of target antibodies or antibody fragments from cell culture supernatants, fermentation broths, and other complex biological samples. Unlike conventional Protein A-based purification, which primarily captures antibodies through recognition of the Fc region, CH1CaptureXP Affinity Resin FF does not require an intact Fc region and is therefore particularly suitable for the purification of bispecific antibodies, human Fab, F(ab')₂, selected antibody fragments, and other CH1-containing antibody formats. The resin is suitable for the purification of human IgG1, IgG2, and IgG4, providing a versatile solution for the purification of different antibody molecules and antibody fragments. Among these subclasses, the resin exhibits the strongest binding performance and highest binding capacity toward human IgG1, enabling efficient antibody purification. With excellent binding selectivity, CH1CaptureXP Affinity Resin FF enables efficient target protein capture and recovery while helping to minimize the co-purification of nonspecific proteins and impurities such as free light chains (FLCs), thereby facilitating the recovery of highly purified antibodies and antibody fragments. The resin is suitable for laboratory-scale antibody purification, antibody fragment preparation, and downstream process development. |
| Key Features |
● Specifically recognizes the CH1 region of the antibody heavy chain, enabling highly selective capture while minimizing the co-purification of non-specific proteins, free light chains, and other impurities. ● Does not depend on an intact Fc region, making it an alternative capture medium for antibody molecules that are not suitable for Protein A-based purification. ● Exhibits excellent alkali stability and good reusability. |
| Formulation | 50% settled resin suspended in PBS (pH 7.4) containing 0.1% ProClin 300 |
| Matrix | 4% highly cross-linked agarose |
| Average Particle Size | 45-165 μm |
| Pressure | ≤0.3 Mpa |
| Ligand | immobilized CH1-specific binder |
| Binding Capacity | ≥40 mg hIgG/mL settled resin; ≥40 mg hIgG-Fab/mL settled resin (depending on flow rate, bed height, and residence time) |
| Elution Method | 0.1 M NaAc (pH 3.5) |
| Reuse Capability | ≥100 purification cycles when regenerated and stored according to the recommended procedures. |
| Application |
Laboratory-scale antibody purification Antibody fragment preparation Downstream process development |
| Storage | Stored at 2°C~8°C. Do not store at 0°C or below 0°C to avoid freezing the resin. |
Binding capacity of CH1CaptureXP Affinity Resin FF (GenScript, L01165) was compared with commercial affinity resins T and P using a panel of IgG and Fab molecules under identical purification conditions. »
Purity of a panel of IgG and Fab molecules purified using CH1CaptureXP Affinity Resin FF (GenScript, L01165) was compared with commercial affinity resins T and P under identical purification conditions. Samples were analyzed by SDS-PAGE under non-reducing and reducing conditions. »
Dynamic binding capacity (DBC) at 10% breakthrough of CH1CaptureXP Affinity Resin FF (GenScript, L01165) as a function of residence time. DBC was determined using purified Omalizumab (IgG1) and Omalizumab-Fab at a protein concentration of 2 mg/mL. »
Binding capacity of CH1CaptureXP Affinity Resin FF (GenScript, L01165) was evaluated after repeated regeneration with 0.1 M NaOH. The resin retained ≥80% of its initial binding capacity after 100 regeneration cycles. »
| Terms of Use |
Product is provided under a free, non-transferable, limited license to use the Product solely in the manner described in its accompanying Product documentation (including, without limitation, any instructions for use, user manuals, certificates of analysis (CoA), datasheets, labels, and other written or electronic materials supplied or referenced by GenScript) and solely for the stated purpose. Any other use may require a separate license from GenScript. This limited, non-transferable license is granted upon the purchase of the Product from GenScript or its authorized distributors. This provision shall not be construed as a warranty of merchantability or fitness for a particular purpose or commercial use. Any reproduction, distribution, modification, reverse engineering, cloning, reverse cloning, sequence determination, sublicensing, or use of the Product to develop competing products or services is prohibited without GenScript’s prior written consent. Third-Party Trademark Acknowledgement All product and company names mentioned in this manual are trademarks or registered trademarks of their respective owners, including but not limited to: ÄKTA, a trademark of Global Life Sciences Solutions USA LLC and its affiliated companies doing business as Cytiva; Triton, a trademark of The Dow Chemical Company; and Tween, a trademark of Croda International Plc. These trademarks are used solely for the purpose of identifying compatible reagents, products, or technologies. GenScript is not sponsored by, affiliated with, or endorsed by the owners of these trademarks. |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
