| ￥8430 在线订购包邮(港澳台除外) | |
| L01161-1 | |
|
|
|
|
|
|
|
|
|
| 咨询产品问题 | |
| Product description |
Anti-DYKDDDDK G2 Affinity Resin FF is a high-performance protein affinity purification medium developed based on a highly specific FLAG® binder. It is specifically designed for the highly selective capture and purification of recombinant proteins containing a FLAG® tag. The product specifically binds to the FLAG® tag on the target protein through an immobilized FLAG® binder, enabling efficient capture of FLAG®-tagged proteins from cell culture supernatants, bacterial lysates, yeast, and other complex biological samples. Compared with the first-generation FLAG® affinity purification medium, this product features a significantly higher binding capacity, allowing larger volumes or higher concentrations of samples to be processed with less resin. This improves purification efficiency while reducing resin consumption and the associated cost per sample. The resin exhibits excellent binding selectivity and high target protein recovery, effectively minimizing the co-purification of non-specific proteins and enabling the recovery of highly purified FLAG®-tagged proteins. The product is compatible with commonly used protein purification buffers and supports mild elution conditions, helping to preserve the structural integrity and biological activity of the target protein. Anti-DYKDDDDK G2 Affinity Resin FF is suitable for recombinant protein purification, high-throughput protein screening, laboratory-scale protein preparation, and downstream purification process development. |
| Key Features |
● High binding capacity (10–25 mg/mL), 5–10 times higher than that of the first-generation FLAG® affinity purification medium. ● High specificity with low non-specific background binding. ● Compatible with gravity-flow chromatography, FPLC (Fast Protein Liquid Chromatography), and automated purification systems. |
| Formulation | 50% settled resin suspended in Glycerol containing 0.1% ProClin300 |
| Matrix | 4% highly cross-linked agarose |
| Average Particle Size | 45-165 μm |
| Pressure | 0.3 Mpa |
| Ligand | DYKDDDDK binder |
| Binding Capacity | 10-25 mg protein/mL settled resin (Actual binding capacity may vary depending on the target protein, tag position and molecular size) |
| Elution Method |
Acid Elution: 0.1 M Gly-HCl, pH 3.0; 0.1 M Gly-HCl, pH 3.5 Alkaline Elution: 0.1 M Tris, pH 12.0 Peptide Competition Elution: 1 mg/mL DYKDDDDK peptide |
| Reuse Capability | 10-20 purification cycles when regenerated and stored as recommended. |
| Reagents Compatibility | Compatible with commonly used lysis reagents. |
| Application |
Recombinant protein purification High-throughput protein screening Laboratory-scale protein preparation Downstream purification process development |
| Storage | Stored at -20°C. |
Binding capacity of Anti-DYKDDDDK G2 Affinity Resin FF (GenScript, L01161) was compared with commercial affinity resins T, SL, and S, as well as GenScript Anti-DYKDDDDK G1 Affinity Resin (GenScript, L00432), using a diverse panel of DYKDDDDK-tagged proteins. Anti-DYKDDDDK G2 Affinity Resin FF exhibited substantially higher binding capacity across all proteins tested compared with the commercial resins. »
A DYKDDDDK tagged Fab molecule purified using Anti-DYKDDDDK G2 Affinity Resin FF (GenScript, L01161) was characterized by SDS-PAGE and SEC-HPLC. The purified Fab achieved 95% purity by SDS-PAGE and 98% purity by SEC-HPLC, with an endotoxin level of <0.049 EU/mg. »
Anti-DYKDDDDK G2 Affinity Resin FF (GenScript, L01161) was evaluated using DYKDDDDK-tagged proteins with different molecular weights and tag positions. The purified proteins showed purity comparable to those obtained using commercial DYKDDDDK affinity resins and GenScript Anti-DYKDDDDK G1 Affinity Resin (GenScript, L00432). »
Anti-DYKDDDDK G2 Affinity Resin FF (GenScript, L01161) demonstrated excellent regeneration performance and operational stability. After 12 reuse cycles, the resin retained 94% regeneration efficiency, outperforming the other commercial DYKDDDDK affinity resins.
Note: The test was performed using a gravity-flow column, and each resin was regenerated according to the respective manufacturer’s recommended protocol. For Anti-DYKDDDDK G2 Affinity Resin FF, 10 CV of 0.01 M NaOH was used for regeneration. »
Anti-DYKDDDDK G2 Affinity Resin FF (GenScript, L01161) demonstrated excellent reusability and regeneration stability. When evaluated on an ÄKTA system using 0.01 M NaOH for regeneration at 15 CV, the resin maintained a regeneration efficiency of >80% after 10 reuse cycles. »
| Terms of Use |
Product is provided under a free, non-transferable, limited license to use the Product solely in the manner described in its accompanying Product documentation (including, without limitation, any instructions for use, user manuals, certificates of analysis (CoA), datasheets, labels, and other written or electronic materials supplied or referenced by GenScript) and solely for the stated purpose. Any other use may require a separate license from GenScript. This limited, non-transferable license is granted upon the purchase of the Product from GenScript or its authorized distributors. This provision shall not be construed as a warranty of merchantability or fitness for a particular purpose or commercial use. Any reproduction, distribution, modification, reverse engineering, cloning, reverse cloning, sequence determination, sublicensing, or use of the Product to develop competing products or services is prohibited without GenScript’s prior written consent. Third-Party Trademark Acknowledgement All product and company names mentioned in this manual are trademarks or registered trademarks of their respective owners, including but not limited to: FLAG® , a trademark of Merck KGaA, Darmstadt, Germany; ÄKTA, a trademark of Global Life Sciences Solutions USA LLC and its affiliated companies doing business as Cytiva; Triton, a trademark of The Dow Chemical Company; and Tween, a trademark of Croda International Plc. These trademarks are used solely for the purpose of identifying compatible reagents, products, or technologies. GenScript is not sponsored by, affiliated with, or endorsed by the owners of these trademarks. |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
