| ￥3024 在线订购包邮(港澳台除外) | |
| L00905-1 | |
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| 咨询产品问题 | |
| Product description |
Camelid VHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx) is a peptide chain
about 110 amino acids in length, containing one variable domain of a heavy-chain antibody (a VHH fragment).
Like a whole antibody, the camelid VHH antibody is able to bind selectively to a specific antigen. This singledomain antibody has a broad range of applications in biotechnical and therapeutic research fields due to its small size, simple production and high affinity. GenScript’s MonoRab™ Anti-Camelid VHH Affinity Resin (Cat. No. L00905) is designed for purification of Camelid VHH single domain antibody from commonly used protein expression systems such as bacteria, yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically bind to the Anti-Camelid VHH monoclonal antibody coupled to the resin. Nonspecific binding reactions are eliminated by stringent washing steps, and the purified Camelid VHH single domain antibody is eluted with a high recovery rate. Table 1 lists the main characteristics of GenScript’s MonoRab™ Anti-Camelid VHH Affinity Resin. |
| Key Features |
● Small size, simple production and high affinity ● Can be recycled at least 5 times with no loss of binding capability ● Can be reused up to 10 times with minimum loss of binding capability ● Acid buffer elution (pH 2.5) |
| Formulation | 50% settled resin in TBS with 0.02% sodium azide |
| Matrix | 4% cross-linked agarose |
| Average Particle Size | 90 μm (45-165 μm) |
| Ligand | MonoRab™ Rabbit Anti-Camelid VHH Antibody, mAb |
| Binding Capacity | Approximately 2-4 mg Camelid VHH protein per mL settled resin (depending on amino acid sequence of different Camelid VHHs) |
| Elution Method | Acid buffer elution (pH 2.5) |
| Reuse Capability | When stored at 2 - 8° C, the resin can be recycled at least 5 times with no loss of binding capability. The resin can be reused up to 10 times with minimum loss of binding capability. |
| Reagents Compatibility | Compatible with commonly used bacterial lysis reagents. |
| Application | purification of Camelid VHH single domain antibody |
| Storage | Stored at 2°C~8°C. Do not freeze. |
图 1. 从大肠杆菌裂解液中纯化驼科 VHH#1 蛋白
泳道：
1. 蛋白Marker
2. 完整细胞裂解液产物
3. 流穿液
4.洗涤流穿液（第一次）
5. 洗涤流穿液（最后一次）
6. 洗脱有目的驼科VHH 单域抗体-1 的洗脱液（分子量 = 15 kDa 单体和 30 kDa 二聚体） »
图 2. 从大肠杆菌裂解液中纯化驼科VHH#2 蛋白
1．蛋白Marker
2．流穿液1
3．流穿液2
4．洗脱有目的VHH2的洗脱液-1
5．洗脱有目的VHH2的洗脱液-2
6．空白洗脱液对照
7．完整细胞裂解液产物 »
| Terms of Use | This product may be the subject of one or more patents filed by GenScript Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer should not sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. For commercial use, please contact GenScript at product@genscript.com. |
For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.
