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Enceed™ T cell Activation, human

The Enceed T cell Activation reagent induces T cell activation and expansion in enriched T cells or PBMCs. The Enceed T cell Activation reagent is composed of biodegradable matrix-coated nanoparticles conjugated with mouse anti-human CD3 and mouse anti-human CD28 antibodies. The small sized particulate structure of the activation reagent enables its easy removal by media washing or centrifugation.
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L00899-0.5

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产品概述
Product Description The Enceed T cell Activation reagent induces T cell activation and expansion in enriched T cells or PBMCs. The Enceed T cell Activation reagent is composed of biodegradable matrix-coated nanoparticles conjugated with mouse anti-human CD3 and mouse anti-human CD28 antibodies. The small sized particulate structure of the activation reagent enables its easy removal by media washing or centrifugation.
The recommended titer of 1:200 has been found to achieve efficient stimulation of majority of T cell subsets. For special applications, the optimal stimulation titer should be determined experimentally. Excessive amount of the Enceed T cell Activation reagent may result in over-activation of T cells risking activation-induced cell death. Typically, the cells reach the exponential growth phase over 5 days and keep optimal expansion up to 13 days. Restimulation after 13 days can be performed for longer cultivation. The Enceed T cell Activation reagent can be used together with human IL-2, other cytokines such as human IL-15 and IL-7 can also be added for more efficient activation.
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产品属性
Format Supplied in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and Poloxamer 188.
Components 1 mL or 0.5 mL Enceed T cell Activation, human
Nanobeads conjugated to monoclonal anti-human CD3 and anti-human CD28 antibodies
Volume
Cat. No.SizeCapacity
L00899-0.5500 μLUp to 1×108 T cells
L00899-11 mLUp to 2×108 T cells
Storage & Stability Store at 2-8 °C. Do not freeze.
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图例
Enceed™ T Cell Activation, Human

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Enceed™ T Cell Activation, Human

T cells enriched by CytoSinct™ CD4 and CytoSinct™ CD8 nanobeads were activated by Enceed™ T cell Activation reagent. After 2 days, T cells were stained with CD25-PE and CD69-APC antibodies. Expression of CD25 and CD69 was analyzed by flow cytometry (Figure A). »

Enceed™ T Cell Activation, Human

T cells stained with carboxy fluorescein succinimidyl ester (CFSE) were stimulated by Enceed™ T cell Activation. After 7 days, proliferation was tested by flow cytometry. »

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.