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ssDNA_RAB11A_GFP

ssDNA_RAB11A_GFP
KIOK9
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Description ssDNA_RAB11A_GFP HDR donor template is high purity GenExactTMssDNA designed for inserting GFP into targeted RAB11A locus. 

Advantages of GenExactTM ssDNA donor template:
✔ Minimal cytotoxicity
✔ Precise knock-in, minimized off-target effect
✔ High purity and sequence verified
✔ Research to GMP grade
Learn More>>> https://www.genscript.com/new-single-stranded-dna-synthesis-service.html

This ssDNA HDR donor template works in combination with SafeEdit Rab11a sgRNA and Cas9 protein.


[Reference] Roth. et al. Reprogramming human T cell function and specificity with non-viral genome targeting. Nature 2018, 559(7714): 405-409.
Quantity 50ug
Storage Condition Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Quality Specifications
Item Specifications
Purity (Agarose gel electrophoresis) Single band
Sequence accuracy (Sanger sequencing) 100% sequence alignment
Optical density (Spectrophotometry) 260 nm/230 nm ≥ 2.0; 260 nm/280 nm = 1.8~2.0
Bioburden No colony formation
Endotoxin (Qualitative TAL assay) ≤ 10 EU/mg
Protein Residue (Micro BCA Protein Assay Kit ) ≤50ug/mg

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