dsDNA_RAB11A_GFP_CTS HDR donor template is high purity GenWandTM closed-end linear ssDNA designed for inserting GFP into targeted RAB11A locus.
Advantages of GenWandTM dsDNA donor template:
✔ Covalently closed ends protection for better accuracy
✔ More suitable for scale up
✔ High purity and sequence verified
✔ Research to GMP grade
Learn More>>> https://www.genscript.com/genwand-double-stranded-dna-synthesis-service.html
This design also includes Cas9 Targeting Sequence (CTS) for improved gene knock-in efficiency. CTS allows for the co-delivery of donor DNA and sgRNA/Cas9 complex into the nucleus target gene site, therefore increasing HDR rate. More info for CTS design can be found in this paper collaborated with Dr. Marson at Gladstone and UCSF.
This dsDNA HDR donor template needs to work in combination with SafeEdit Rab11a sgRNA and Cas9 protein.
[Reference] Roth. et al. Reprogramming human T cell function and specificity with non-viral genome targeting. Nature 2018, 559(7714): 405-409.
[Reference] Shy, et al. Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing. bioRxiv 2021.09.02.458799.
|Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles.