目录产品 » Human Recombinant SARS-CoV-2 Spike Stable Cell Line
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CHO-K1/Spike Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human SARS-CoV-2 spike protein on their surface. The surface expression of SARS-CoV-2 spike protein is validated by FACS analysis. This stable cell line product is designed for screening antibodies against the SARS-CoV-2 spike protein, as well as measuring binding affinity and stability of antibody-based biologics that bind with spike protein. GenScript also offers spike protein expressing HEK293T stable cell line (Cat. No. M00804) for SARS-CoV-2 study.
询价
M00803

产品简介
Product Description Recombinant CHO-K1 cells stably overexpress coronavirus SARS-CoV-2 spike protein on the surface. The surface expression of SARS-CoV-2 spike protein is validated by FACS analysis. This stable cell line product is designed for screening antibodies against SARS-CoV-2 spike protein, as well as for measuring binding affinity and stability of antibody based biologics that bind with spike protein. GenScript also offers spike protein expressing HEK293T stable cell line (Cat. No. M00804) for SARS-CoV-2 study.
Culture Properties Adherent.
Stability Stability: Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
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培养条件
Culture Medium Ham's F-12K (Kaighn's), 10% FBS, 8 μg/ml puromycin (Cat. No. A11138-03, Life Technologies)
Complete Growth Medium Ham's F-12K (Kaighn's) (Cat. No. 21127, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
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图例
CHO-K1/Spike Stable Cell Line

Figure 1: FACS analysis of cell surface expression of SARS-CoV-2 spike protein on CHO-K1 cells. CHO-K1/Spike cells (red) and negative control CHO-K1 cells (grey) were probed using ACE-2-Fc Chimera, Human (Cat. No. Z03516, GenScript) and Alexa Fluor 647 conjugated Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Cat. No. A-21445, Thermo Fisher). »

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.