Product Description | Recombinant Daudi cells stably overexpress human programmed cell death protein 1 (PD-1) on the surface. The surface expression of PD-1 is validated by FACS analysis. |
Culture Properties | Suspension. |
Stability | Stability: Stable through more than 15 passages with no significant changes in assay performance or expression profile. |
Size | Two vials of frozen cells (>1×106 per vial in 1 mL) |
Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
Culture Medium | RPMI 1640 Medium, 20% FBS, 0.5 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 1% Sodium Pyruvate (Cat. No. 11360-070, Life Technologies) |
Complete Growth Medium | RPMI 1640 medium, 20% FBS |
Freeze Medium-DATA | 90% FBS (Cat. No. 10099-141, Life Technologies), 5% RPMI 1640 medium (Cat. No. 22400, Life Technologies), 5% DMSO (Cat. No. D2650, Sigma) |
Figure 1. FACS analysis of PD-1 expression in Daudi/PD-1 cells.
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.