| 询价 | |
| M00601 | |
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| Product Description | Recombinant CHO - K1 cells stably overexpress human cell - type natural killer cells Fc gamma receptor IIc1 (FcγRIIc/CD32C) on the surface. The surface expression of CD32C 13Gln is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies and Fc fusion proteins, or antibody based biologics binding with CD32C 13Gln. GenScript also offers other CD32 stable cell line for FcγRII polymorphism study. |
| Culture Properties | Adherent. |
| Stability | Stable through more than 15 passages without significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL). |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Freeze Medium-DATA | 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma). |
Figure 1. FACS analysis of cell surface expression of CD32C 13Gln on CHO-K1/CD32 C 13Gln cells. The CHO-K1/CD32 C 13Gln cells (blue) and the negative control CHO-K1 cells (red) were probed using APC-conjugated mouse anti-human CD32 antibody (Cat. No. 559769, R&D Systems).
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
