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| M00597 | |
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| Product Description | Recombinant CHO-K1 cells stably overexpress human low affinity immunoglobulin gamma Fc region receptor III-A (FcγRIIIa/CD16A 158V) and Fc epsilon receptor Ig (FcER1G) on the surface. The surface expression of CD16A 158V is validated by FACS analysis. |
| Culture Properties | Adherent. |
| Stability | Stable through more than 15 passages without significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL). |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No.10687010, Life Technologies). |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Freeze Medium-DATA | 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma). |
Figure 1. FACS analysis of cell surface expression of CD16A 158V on CHO-K1/CD16A 158V cells. The CHO-K1/CD16A 158V cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-CyTM5 mouse anti-human CD16 (Cat. No. 555408, BD Bioscience).
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
