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CHO-K1/cyno PD-1 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress cynomolgus programmed cell death protein 1 (PD-1) on the surface. The surface expression of PD-1 is validated by FACS analysis.
询价
M00572

Product Description Recombinant CHO-K1 cells stably overexpress Macaca fascicularis programmed cell death protein 1 (PD-1) on the surface. The surface expression of PD-1 is validated by FACS analysis.
Culture Properties Adherent.
Stability Stable through more than 15 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
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Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco).
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco).
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)
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CHO-K1/cyno PD-1 Stable Cell Line

Figure 1. FACS analysis of cell surface expression of cynomolgus PD-1 on CHO-K1/cyno PD-1 cells. The CHO-K1/cyno PD-1 cells (red) and the negative control CHO-K1 cells (blue) were probed using APC-conjugated anti-human CD279 antibody (Cat. No. 329908, Biolegend). »

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.