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| M00539 | |
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| Product Description | Recombinant CHO-K1 cells stably overexpress Homo sapiens TNF receptor superfamily member 18 (GITR) on the surface. The surface expression of GITR is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against human GITR or to measure binding affinity between GITR and anti-GITR antibodies. |
| Culture Properties | Adherent |
| Stability | Stable through more than 16 passages without significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL). |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 6 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Freeze Medium-DATA | 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma) |
Figure 1. FACS analysis of cell surface expression of human GITR on CHO-K1/GITR cells. The CHO-K1/GITR cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human CD357 antibody (Cat. No. 371203, Biolegend). »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
