目录产品 » 稳定细胞系 » Human Recombinant Gonadotropin-releasing Hormone Receptor Stable Cell Line
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CHO-K1/GNRHR/Gα15 Stable Cell Line

The gonadotropin-releasing hormone receptor (GNRHR), also known as the luteinizing hormone releasing hormone receptor (LHRHR), is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Upon activation, the LHRHr stimulates tyrosine phosphatase and elicits the release of LH from the pituitary. Evidence exists showing the presence of LHRH and its receptor in extrapituitary tissues as well as a role in progression of some cancers.
询价
M00426

Synonyms

GnRH receptor, luteinizing hormone-releasing hormone (LHRH) receptor, LHRHR, LRHR, GRHR, GNRHR1, luliberin receptor, GnRH1, Type I GnRHR, LHRH receptor, GnRH I receptor, GH1, gnRH receptor, gnRH-R, gonadotropin-releasing hormone receptor, growth hormone

产品简介
Storage Liquid nitrogen immediately upon delivery
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培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)
Freeze Medium-DATA 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
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图例
CHO-K1/GNRHR/Gα15 Stable Cell Line

Figure 1. Buserelin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GNRHR/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist buserelin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of buserelin (Mean ± SD, n = 2). The EC50 of buserelin on this cell was 20.2 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom »

CHO-K1/GNRHR/Gα15 Stable Cell Line

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