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| M00342 | |
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Human Recombinant VPAC1 Receptor Stable Cell Line
| Storage | Liquid nitrogen immediately upon delivery |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies) |
| Freeze Medium-DATA | 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma) |
Figure 1. VIP-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/VPAC1/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist VIP. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of VIP (Mean ± SD, n = 2). The EC50 of VIP in this cell was 52.2 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
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