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CHO-K1/OXTR Stable Cell Line

Oxytocin is an abundant neuropeptide that exerts a wide spectrum of central and peripheral effects. In the context of human reproduction, oxytocin promotes uterine contractions and lactation. It is the most commonly used drug for labor induction. Oxytocin receptors are expressed in the uterus and in mammary glands, where they mediate functions related to partutition, such as contraction of the uterine myometrium during labor and milk letdown. In addition, oxytocin receptors are expressed in a variety of other peripheral tissues and in the brain, where oxytocin mediates a variety of functions. OXTR antagonists may be promising candidates to prevent preterm labor and dysmenorrheal and its agonists may also be useful for treatment of psychiatric illnesses such as anxiety, drug abuse, sexual dysfunctions, eating disorders, and autism.
询价
M00195

产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human oxytocin receptor (OXTR) on the surface and contain high levels of G protein Gαq and Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent.
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL).
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
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培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)
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图例
CHO-K1/OXTR Stable Cell Line

Figure 1. Oxytocin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OXTR cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to being stimulated with agonist oxytocin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of oxytocin (Mean ± SEM, n = 3). The EC50 of oxytocin on this cell was 0.68 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape. »

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


相关文献
Pierce ML, et al. Comparison of the pharmacologic profiles of arginine vasopressin and oxytocin analogs at marmoset, titi monkey, macaque, and human oxytocin receptors. Biomed Pharmacother. (2020)
PierceMarsha L, et al. A Comparison of the Ability of Leu- and Pro-Oxytocin to Regulate Intracellular Ca and Ca-Activated K Channels at Human and Marmoset Oxytocin Receptors. Mol. Pharmacol. (2019)