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目录产品 » 稳定细胞系 » Human Recombinant KiSS1-derived Peptide Receptor Stable Cell Line
CHO-K1/KiSS1 Stable Cell Line

Figure 1: Kisspeptin-10-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/KiSS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with KiSS1 receptor agonist, Kisspeptin-10. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of LPA (Mean ± SD, n = 2). The EC50 of LPA on CHO-K1/KiSS1 cells was 14.2 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/KiSS1 Stable Cell Line

The KiSS1-derived peptide receptor (also known as GPR54 or the Kisspeptin receptor) is a Gq-coupled receptor which binds the peptide hormone kisspeptin (metastin). The KiSS1 gene inhibits metastatic activity of melanoma and other tumor cell lines, and clinical evidence supports a role for KiSS1 in inhibition of metastasis in human cancer. Kisspeptins and GPR54 also play a central role in hypothalamic regulation of puberty, by directly governing the release of gonadotropin-releasing hormone from the hypothalamus. In addition, mutations in GPR54 in mice and humans result in hypogonadotropic hypogonadism.
M00190
¥85000

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KISS1R, GPR54, Metastin receptor, AXOR12, OT7T175

Applications Functional assay for KiSS1 receptor

Storage Liquid nitrogen immediately upon delivery
Species Human

Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

  • CHO-K1/KiSS1 Stable Cell Line
  • CHO-K1/KiSS1 Stable Cell Line

    Figure 1: Kisspeptin-10-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/KiSS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with KiSS1 receptor agonist, Kisspeptin-10. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of LPA (Mean ± SD, n = 2). The EC50 of LPA on CHO-K1/KiSS1 cells was 14.2 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is the response and starts at Bottom and goes to Top with a sigmoid shape.