CHO-K1/CCKB Stable Cell Line
Human Recombinant CHO-K1/CCKB Stable Cell Line stably overexpress the Cholecystokinin Receptor gene (CCKB). This stable cell line product is designed for measuring the In Vitro function of CCKB.
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| M00154 | |
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| 询价 | |
| M00154 | |
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| Product Description |
Human Recombinant CHO-K1/CCKB Stable Cell Line
stably overexpress the Cholecystokinin Receptor gene (CCKB). This stable cell line product is designed for measuring
the In Vitro function of CCKB. |
| Culture Properties |
Adherent |
| Stability |
Stable through more than 16 passages without significant changes in
assay performance or expression profile. |
| Size |
Two vials of frozen cells (1×106 per
vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon receipt. Thaw and
recover cells within one year from the date received. |
| Culture Medium |
Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01,
Life Technologies) |
| Complete Growth Medium |
Ham’s F-12K (Kaighn’s), 10% FBS |
| Freeze Medium-DATA |
Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Gibco), 45% FBS (Cat. No. A5669701,
Gibco),
10% DMSO (Cat. No. D2650, Sigma) |
Figure 1: CCK8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CCKB cells.
The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to stimulation with CCKB agonist, CCK8. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of CCK8 (mean ± SD, n = 3). The EC50 of CCK8 on CHO-K1/CCKB cells was 0.945 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.
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For research use only. Not intended for human or animal
clinical trials, therapeutic or diagnostic use.
