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| M00150 | |
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| Product Description | Recombinant CHO-K1 cells stably overexpress human endothelin receptor type B (EDNRB) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways. |
| Culture Properties | Adherent |
| Stability | Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 10% FBS |
| Freeze Medium-DATA | 45% culture medium, 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma) |
Figure 1. Endothelin 1-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/EDNRB cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to being stimulated with agonist endothelin 1. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of endothelin 1 (Mean ± SEM, n = 3). The EC50 of endothelin 1 on this cell was 43.44 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is ∆RFU and starts at Bottom and goes to Top with a sigmoid shape. »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
