HEK293/H1 Stable Cell Line
Recombinant HEK293 cells stably overexpress human histamine receptor 1 (H1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
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| 询价 | |
| M00131 | |
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| Product Description | Recombinant HEK293 cells stably overexpress human histamine receptor 1 (H1) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways. |
| Culture Properties | Adherent. |
| Stability | Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL). |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | DMEM, 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies) |
| Complete Growth Medium | DMEM, 10% FBS |
| Freeze Medium-DATA | 50% DMEM (Cat. No. 10566-016, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 5% DMSO (Cat. No. D2650, Sigma). |
Figure 1. Histamine-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/H1 cells. The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to being stimulated with agonist Histamine. The intracellular calcium change was normalized and measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of Histamine (Mean ± SEM, n = 3). The EC50 of Histamine on this cell was 0.65 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.
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Figure 2. Dose dependent stimulation of intracellular IP-One accumulation upon treatment with Histamine in HEK293/H1 cells. d2 acceptor fluorophore-labeled IP-One (Cat. No. 62IPAPEB; Revvity) and intracellular IP-One in HEK293/H1 cells competitively bind with Europium Cryptate-labeled anti-IP-One antibody. The FRET signal decreases as the intracellular IP-One concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of Histamine on HEK293/H1 cells was 19.73 nM. »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
