HRP-Protein G

Protein G, a cell surface protein of Group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A. It contains 3 IgG binding domains and is engineered to delete albumin-binding region. HRP has been covalently coupled to Protein G to produce the HRP-Protein G, which can be used in detection systems such as immunoblotting, ELISA, immunoperoxidase electron microscopy and immunohistochemistry.

价格	￥750 在线订购包邮(港澳台除外)
产品编号	M00090
规格	1 mg 大单询价
数量
参考货期


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应用指导产品属性图例技术背景

应用指导

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase antibody based enzymatic assays requiring lot-to-lot consistency. Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including primary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Dot blot	1:2,000-1:10,000
Western blot	1:2,000-1:10,000
ELISA	1:10,000-1:50,000
Immunohistology	1:1,000-1:10,000

产品属性

Concentration	1 mg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal.
Reconstitution	Reconstitute the lyophilized power with 1.0 ml of deionized water to a final HRP-Protein G concentration of 1 mg/ml.
Storage	The product remains stable in lyophilized form for 1 year if stored at 2-8°C, -20°C, or lower from the date of receipt. For longer storage, -20°C or below is recommended. The reconstituted product can be stored for 2-3 weeks at 2-8°C or for 1 year at -20°C or below. Avoid repeated freezing and thawing cycles.

图例

技术背景

Target Background

For research use only. Not intended for human and animal therapeutic or diagnostic use.