T7 RNA Polymerase ELISA kit
T7 RNA Polymerase ELISA kit is used for detection and quantitative determination of T7 RNA Polymerase in the samples.
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| L01110 | |
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| L01110 | |
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| Product Description | The kit is used for detection and quantitative determination of T7 RNA Polymerase in the samples. T7 RNA Polymerase ELISA kit uses the double antibody sandwich method: The micro plate has been pre-coated with anti-T7 RNA polymerase antibody. The sample is added and incubated with horseradish peroxidase (HRP) labeled antibody to form antibody-antigen-enzyme-labeled antibody complex. After washing, then TMB substrate solution is added and catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The depth of the color is positively correlated with the T7 RNA polymerase content in the sample. The absorbance (OD value) is measured at 450 nm. | ||||||||||||||||||||
| Linearity Range | 0.25-32 ng/mL | ||||||||||||||||||||
| Detection Range |
Lower limit of detection: ＜0.1 ng/mL Lower limit of quantitation: 0.25 ng/mL |
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| Precision | Intra-assay CV≤10%, Inter-assay CV≤15% | ||||||||||||||||||||
| Recovery | 80%-120% | ||||||||||||||||||||
| Specificity | only specific recognition of T7 RNA polymerase, other IVT tool enzymes do not interfere with the measurements. | ||||||||||||||||||||
| Storage | Unopened kit should be stored at 2-8°C for stability in shelf life. | ||||||||||||||||||||
| Kit Contents |
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
