Product Description |
CytoSinct CD4 Nanobeads, human is used for in
vitro enrichment of CD4+ cells from fresh or frozen peripheral
blood mononuclear cells (PBMCs), leukapheresis products, or single-cell
suspension based on the surface expression of human CD4. To begin the isolation, Nanobeads are added to the cells. The cells/beads mixed suspension is loaded onto a CytoSinct column placed in the CytoSinct magnet, or other compatible columns with a magnet. The CD4+ cells labeled with Nanobeads are retained within the column and unlabelled cells (CD4- cells) are flushed out during the wash step. After removing the column from the magnetic field, the retained CD4+ cells can be eluted as the positively selected cell fraction. |
Format |
Supplied
in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and
Poloxamer 188. |
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Components |
1 mL or
0.5 mL CytoSinct CD4 Nanobeads, human Nanobeads conjugated to monoclonal anti-human CD4 antibodies (isotype: mouse IgG1). |
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Volume |
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Storage & Stability |
Store at 2 - 8 °C. Do not freeze. |
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CD4+ T cells were enriched from human PBMCs. CD4+ cells were stained with anti-CD4 (clone: SK3) APC and gated on Live/hCD45+. »
For
research use only. Not intended for human or animal clinical trials,
therapeutic or diagnostic use.