• 中文
  • 登录
  • 联系我们
×
目录产品 » GenCRISPR™/Cas9 基因编辑相关产品 » GenCRISPR™ Cas9 Enzymes » GenCrispr sgRNA Screening Kit

GenCrispr sgRNA Screening Kit

Description Cas9 Nuclease NLS, S. pyogenes, is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The design of single guide RNA (sgRNA) is dependent on the target region close to the PAM site. Even if you pick a target sequence that fulfills all of the described requirements, sgRNA specificity and activity is unpredictable. Therefore, it is often recommended that multiple, different sgRNAs be designed to target a gene of interest. The GenCrispr sgRNA Screening Kit provides a simple, reliable, and rapid method for assessing sgRNA efficiency before cell transduction, allowing you to identify the highly effective CRISPR sgRNA.

Components Amount
30-reaction kit 100-reaction kit
GenCrispr Cas9 Nuclease 15 μL 50 μL
10X Reaction Buffer 100 μL 300 μL
Positive Control sgRNA 3 μg 10 μg
Positive Control Substrate 60 μL 200 μL
RNase-free water 1 mL 1 mL

Storage Store at -20°C upon receipt. positive control sgRNA should be aliquoted and stored at -80°C, to avoid repeated freeze/thaw cycles.
Note Research purposes only.

Evaluate the efficiency of sgRNAs for CRISPR/Cas9 in vitro studies and identify the highly effective sgRNAs before cell transfection.

  • GenCrispr SgRNA Screening Kit
  • GenCrispr SgRNA Screening Kit




请点击此处查找更多相关文献






更多产品文件类型 >>

产品编号: L00689
规格
价格
1187258 L00689-30 30 reactions ¥1992.00
1187259 L00689-100 100 reactions ¥4800.00