GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.
|Cat. No.||Product Name
|L00683-12||High Affinity Ni-charged Resin FF prepacked column (1mlx2)||1mlx2||prepacked column|
|L00683-51||High Affinity Ni-charged Resin FF prepacked column (5mlx1)||5mlx1|
|L00683-55||High Affinity Ni-charged Resin FF prepacked column (5mlx5)||5mlx5|
Store in 20% ethanol at 2°C to 8°C. Do not freeze.
|Binding capacity||>40 mg of histidine-tagged protein /ml settled resin|
|Matrix spherical||6% highly cross-linked agarose |
|Average particle size||90 μm (45-165 μm)|
|Storage solution||20% ethanol |
|Storage and Stability||18 months when stored unopened at 2-8 °C|
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).
|Denaturants||Detergents||Reducing agents||Salts ||Others|
|6 M Gu·HCl||2% Triton X-100 ||20 mM β-ME||4 M MgCl2||50% glycerol |
|8 M Urea ||2% Tween 20 ||1 mM DTT ||5 mM CaCl2||20% ethanol |
|1% CHAPS||2 M NaCl||1 mM EDTA|