GenScript Protein G Resin FF is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin FF is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein G Resin FF Prepacked Column.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
|Cat. No.||Product Name
|L00681-12||Protein G Resin FF (1mlx2)||1mlx2||50% slurry|
|L00681-51||Protein G Resin FF (5mlx1)||5mlx1||50% slurry|
|L00681-55||Protein G Resin FF (5mlx5)||5mlx5||50% slurry|
Store in 20% ethanol at 2°C to 8°C. Do not freeze.
|Ligand||Recombinant Streptococcal protein G lacking the albumin-binding sites expressed in E. coli|
|Number of IgG binding sites per ligand||3|
|M.W. of ligand||Approximately 22 kDa|
|PI of ligand||4.69|
|Degree of substitution||Approximately 2 mg protein G/ml settled resin|
|Static binding capacity||≥ 20 mg human IgG/ml settled resin|
|Matrix spherical||4% highly cross-linked agarose|
|Average particle size||90 μm (45-165 μm)|
|Recommended flow rate ||50-300cm/h|
|Storage solution||20% ethanol |
|Storage and Stability||18 months when stored unopened at 2-8 °C|
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).