Anti-His Affinity Resin

GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein. It is composed of agarose and conjugated THETM anti-His monoclonal antibody. His-tagged protein in the crude samples can bind to the anti-His monoclonal antibodies coupled with the agarose, and the nonspecific proteins can be eliminated by washing. As a result, the His-tagged protein will finally be eluted with high recovery. Moreover, this product can be used for immunoprecipitation of His-tagged protein.

价格	￥3990
产品编号	L00439-1
规格	1 ml Resin (Total 2 ml) 5 ml Resin (Total 10 ml) 大单询价
数量

产品概述产品属性图例

产品概述

Product Description

GenScript Anti‐His Affinity Resin is designed for purification of His‐tagged protein that is difficult for single Ni‐NTA Agarose purification. This product is made from THE™ anti‐His monoclonal antibody conjugated to agarose resin. It specifically binds to His‐tagged proteins in the samples. Proteins nonspecifically binding to the resin can be eliminated by washing. Finally, His‐tagged protein can be eluted by different methods. In addition to that, the resin can be used for immunoprecipitation of His‐tagged proteins.

产品属性

Product Content	50% resin in TBS with 0.02% sodium azide
Beads Structure	4% agarose
Beads Size	45-165 μm, averagely 90 μm
Coupled Antibody	THE™ His Tag Antibody, mAb, Mouse (Cat.No A00186)
Binding Capacity	More than 0.6 mg of His‐tagged protein per ml of settled resin
Specificity	N‐terminal/C‐terminal/internal /4 x His/5 x His/6 x His‐tagged proteins
Elution Method	Acid, alkaline and peptide competitive elutions
Repeat Usage	Generally the resin can be recycled at least 4 times. If maintained properly, the resin may reach 10 recycle times with minor binding capability loss
Reagents Compatibility	Compatible with mostly used reagents at certain concentrations
Consistency	Lot‐to‐lot consistency proved by strict production procedure
Storage & Stability	This product is stable for 1 year if stored at 2°C~8°C from the date of manufacture. Do not store at 0°C or below 0°C to avoid freezing the resin.

图例

SDS-PAGE analysis of protein purified by Anti-His Affinity Resin or Ni-NTA Agarose.
Test result demonstrated that the Anti-His Affinity Resin can purify the His-tagged proteins with high purity from
different samples in which Ni-NTA Agarose had failed. »

SDS-PAGE analysis of protein eluted from Anti-His Affinity Resin.
M. Marker
1. His-tagged protein using the acid elution procedure
2. His-tagged protein using the alkaline elution procedure
3. His-tagged protein by competitive elution procedure
Test result demonstrated that the His-tagged protein can be eluted by three different eluents. »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.