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| L00439-1 | |
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| Product description | GenScript Anti‐His Affinity Resin is designed for purification of His‐tagged protein that is difficult for single Ni‐NTA Agarose purification. This product is made from THE™ anti‐His monoclonal antibody conjugated to agarose resin. It specifically binds to His‐tagged proteins in the samples. Proteins nonspecifically binding to the resin can be eliminated by washing. Finally, His‐tagged protein can be eluted by different methods. In addition to that, the resin can be used for immunoprecipitation of His‐tagged proteins. |
| Key Features |
● Composed of agarose and conjugated THE™ anti-His monoclonal antibody ● Specifically binds to His-tagged proteins ● Nonspecific proteins can be eliminated by washing |
| Formulation | 50% resin in TBS with 0.02% sodium azide |
| Matrix | 4% cross-linked agarose |
| Average Particle Size | 90 μm (45-165 μm) |
| Ligand | THE™ His Tag Antibody, mAb, Mouse (Cat.No A00186) |
| Binding Capacity | ≥ 0.6 mg of His‐tagged protein per ml of settled resin |
| Specificity | N‐terminal/C‐terminal/internal /4 x His/5 x His/6 x His‐tagged proteins |
| Elution Method | Acid, alkaline and peptide competitive elutions |
| Reuse Capability | Generally the resin can be recycled at least 4 times. If maintained properly, the resin may reach 10 recycle times with minor binding capability loss |
| Reagents Compatibility | Compatible with mostly used reagents at certain concentrations |
| Application |
purification of His-tagged protein; immunoprecipitation of His-tagged protein |
| Storage | Stored at 2°C~8°C. Do not store at 0°C or below 0°C to avoid freezing the resin. |
»
SDS-PAGE analysis of protein purified by Anti-His Affinity Resin or Ni-NTA Agarose.
Test result demonstrated that the Anti-His Affinity Resin can purify the His-tagged proteins with high purity from
different samples in which Ni-NTA Agarose had failed. »
SDS-PAGE analysis of protein eluted from Anti-His Affinity Resin.
M. Marker
1. His-tagged protein using the acid elution procedure
2. His-tagged protein using the alkaline elution procedure
3. His-tagged protein by competitive elution procedure
Test result demonstrated that the His-tagged protein can be eluted by three different eluents. »
For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.
