ToxinEraser™ Endotoxin Removal Kit

GenScript ToxinEraser™ is an endototoxin removal resin of high efficiency. It is based on the affinity matrix of modified polymyxin B (PMB), which allows highly efficient endotoxin removal. The final endotoxin level can be decreased to less than 0.1 EU/ml with repeat use of ToxinEraser™ endotoxin removal resin. Final removal efficiency may vary depending on the sample type/source.

价格	￥1200
产品编号	L00338
规格	1 Kit 大单询价
数量
TAT

产品简介图例

产品简介

Product Description

GenScript’s ToxinEraser™ is an endototoxin removal resin of high efficiency. It is based on affinity matrix of modified polymyxin B (PMB), which allows highly efficient endotoxin removal. The final endotoxin level can be decreased to less than 0.1 EU/ml with repeated use of ToxinEraser™ endotoxin removal resin. Final removal efficiency may vary depending on the sample type/source.

Key Features

● High stability and high removal efficiency
● High binding capacity: > 2,000,000 EU/ml settled resin
● Fast flow without any constant speed pump
● Reusable up to five times if properly regenerated
● Ready-to-use reagents and materials, such as equilibration and regeneration buffers, collection tubes, etc.

Size

1.5 ml in pre-packed column

Binding capacity

Up to 2,000,000 EU/ml resin

Ligand

Modified PMB (Polymixin B)

pH Stability

pH 5-10

Support Matrix

4% cross-linked agarose, spherical beads

Mean Particle Size

90 μm

Equilibration Buffer

Sodium Phosphate-Buffer, pH 8.0

Regeneration Buffer

Sodium Phosphate-Buffer, pH 8.0; Triton-114

Targets Suitable for Purification

Purified recombinant proteins, peptides, antibodies, polysaccharides, etc.

Tolerable Ionic Strength

0.1-0.5 M NaCl

Performance-compatible Reagents

20% DMSO, 20% ethanol, 20% glycerol;1 M urea, 300 mM imidazole; 0.05% Tween 20, 10 mM DTT.

Storage

2°C to 8°C (Do not freeze.)

Kit Contents

Kit Contents （Cat. No.:L00338）	PK
ToxinEraser™ Endotoxin Removal Resin	1.5 ml pre-packed column
Regeneration Buffer	125 ml
Equilibration Buffer	125 ml
Flow-Speed Control	1
Collection Tubes	1 bag（3 Tubes）
Tips (1 ml)	2 bags（6 Tips/bag）
Protocol	1

图例

The Effect of pH on Removal Efficiency
Fig. 1. Endotoxin removal can be performed in solutions from pH 5 -10 but the highest removal efficiency occurs at pH 7-8. The removal efficiency decreases upon deviation from neutral pH. »

The Effect of Endotoxin Concentration on Removal Efficiency
Fig. 2. Endotoxin removal efficiency is highly affected by and directly proportional to endotoxin concentration. »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

相关文献

1. Hui Guan, et al. Anti-PS IgG Immune Complexes Impair Macrophage Phagocytosis in SLE via LOX-Dependent Oxidative Stress. Journal of Inflammation Research. (2025-08)
2. Yuan Yuan, et al. A Streptococcus pneumoniae endolysin mutant protein ΔA146Ply elicits rapid broad-spectrum mucosal protection in mice via upregulation of GPX4 through TLR4/IRG1/NRF2 to alleviate macrophage ferroptosis. Free Radic Biol Med. (2024-06)
3. Wenlong Xu, et al. Streptococcus pneumoniae endopeptidase O induces trained immunity and confers protection against various pathogenic infections. Clin Immunol. (2024-04)
4. Jingwen Liao, et al. A rational designed multi-epitope vaccine elicited robust protective efficacy against Klebsiella pneumoniae lung infection. Biomed Pharmacother. (2024-04)
5. Yuwei Li, et al. Structure/epitope analysis and IgE binding activities of three cyclophilin family proteins from Dermatophagoides pteronyssinus. Sci Rep. (2023-08)
6. Xi Yu, et al. Lipases secreted by a gut bacterium inhibit arbovirus transmission in mosquitoes. PLoS Pathog. (2022-06)
7. Yanping Hu, et al. Genomic distribution of signal transducer and activator of transcription (STAT) family in colorectal cancer. Hum Cell. (2022)
8. Peipei Zhang, et al. NLRC4 inflammasome-dependent cell death occurs by a complementary series of three death pathways and determines lethality in mice. Sci Adv. (2021-10)
9. Jifeng Zhao, et al. Activation of α7-nAChRs Promotes the Clearance of α-Synuclein and Protects Against Apoptotic Cell Death Induced by Exogenous α-Synuclein Fibrils. Front Cell Dev Biol. (2021-02)
10. Zhixiao Sun, et al. Epithelial-Mesenchymal Transition in Asthma Airway Remodeling Is Regulated by the IL-33/CD146 Axis. Front Immunol. (2020)
11. EsteghamatFatemehsadat, et al. CELA2A mutations predispose to early-onset atherosclerosis and metabolic syndrome and affect plasma insulin and platelet activation. Nat Genet. (2019-08)
12. WangMengqiang, et al. Comparative study of β-thymosin in two scallop species Argopecten irradians and Chlamys farreri. Fish Shellfish Immunol. (2019-03)
13. Hörner M,,, Raute K,,, Hummel B,, Madl J,, Creusen G,, Thomas OS,,, Christen EH, Hotz N,, Gübeli RJ,, Engesser R,, Rebmann B, Lauer J,, Rolauffs B, Timmer J,, Schamel WWA,,,, Pruszak J,, Römer W,,,, Zurbriggen MD, Friedrich C, Walther A,,, Minguet S,,,, Sawarkar R,, Weber W,,., et al. Phytochrome-Based Extracellular Matrix with Reversibly Tunable Mechanical Properties. Adv Mater. (2019)
14. Gao Q,, Hong J,, Xiao X, Cao H, Yuan R, Liu Z,, Chen T., et al. T cell epitope of arginine kinase with CpG co-encapsulated nanoparticles attenuates a shrimp allergen-induced Th2-bias food allergy. Biosci Biotechnol Biochem. (2019)
15. SánchezJorge, et al. Causal Relationship Between Anti-TPO IgE and Chronic Urticaria by and Tests. Allergy Asthma Immunol Res. (2019)
16. Xin Wu, et al. Reversible hydrogels with tunable mechanical properties for optically controlling cell migration. Nano Research. (2018-10)
17. BrangelPolina, et al. A Serological Point-of-Care Test for the Detection of IgG Antibodies against Ebola Virus in Human Survivors. ACS Nano. (2018)
18. WangHui, et al. Der f 31， a novel allergen from Dermatophagoides farinae， activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells. Sci Rep. (2017-08)
19. WangHui, et al. Pplase of Dermatophagoides farinae promotes ovalbumin-induced airway allergy by modulating the functions of dendritic cells in a mouse model. Sci Rep. (2017-02)
20. Dan Li, et al. The effect of local delivery of adiponectin from biodegradable microsphere-scaffold composites on new bone formation in adiponectin knockout mice. J Mater Chem B. (2016-06-01)
21. Wang M, et al. An LRR-only protein representing a new type of pattern recognition receptor in Chlamys farreri. Dev Comp Immunol. (2016-01)
22. Dan Li, et al. The effect of local delivery of adiponectin from biodegradable microsphere–scaffold composites on new bone formation in adiponectin knockout mice. J. Mater. Chem. B,. (2016)
23. Chen T, et al. Adiponectin enhances osteogenic differentiation in human adipose-derived stem cells by activating the APPL1-AMPK signaling pathway. Biochem Biophys Res Commun. (2015-05)
24. Hu H,, Pu Y,, Lu S,, Zhang K, Guo Y,, Lu H,, Li D,, Li X, Li Z, Wu Y,, Tang Z,., et al. The Osteogenesis Effect and Underlying Mechanisms of Local Delivery of gAPN in Extraction Sockets of Beagle Dogs. Int J Mol Sci. (2015)
25. M Wang, et al. A high mobility group box 1 (HMGB1) gene from Chlamys farreri and the DNA-binding ability and pro-inflammatory activity of its recombinant protein. Fish Shellfish Immunol. (2013-12)