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| L00210 | |
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| Product description | GenScript Protein A Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin. The binding capacity will vary depending on several factors such as target antibody, flow rate etc. |
| Key Features |
● optimized coupling for high binding capacity ● Fab region available for binding antigens ● recombinant protein with non-essential domains removed to reduce nonspecific binding |
| Formulation | 50% slurry in 20% ethanol |
| Matrix | 4% cross-linked agarose |
| Average Particle Size | 90 μm (45-165 μm) |
| Ligand | Recombinant Streptococcal protein A expressed in E. coli |
| Number of binding sites per ligand | 5 |
| M.W. of ligand | Approximately 34 kDa |
| PI of ligand | 5.17 |
| Binding Capacity | ＞20 mg human IgG/ml settled resin |
| Degree of substitution | Approximately 2 mg protein A/ml settled resin |
| Application |
purification of classes, subclasses and fragments of immunoglobulins; immunoprecipitation of proteins, protein complexes or antigens |
| Storage |
2-8 °C; Do not freeze. |
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For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.
