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目录产品 » GenCRISPR™/Cas9 基因编辑相关产品 » GenCRISPR™ Cas9 Enzymes » GenCRISPR™ Cas13a (C2c2) Nuclease

GenCRISPR™ Cas13a (C2c2) Nuclease

GenCRISPR Cas13a (C2c2) Nuclease is an RNA-guided, RNA-targeting CRISPR enzyme, commonly referred to as C2c2. This enzyme is a member of Type VI CRISPR family containing a single protein effector with two HEPN domains. The Cas13a exhibits two RNase activities. The first activity, a cis- sequence-specific RNA guided cleavage, activates the secondary RNase activity of the enzyme, a non-specific trans- ribonuclease activity. This Cas13a protein can be applied to detect single RNA molecules with proven high specificity.
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GenCRISPR Cas13a (C2c2) Nuclease is an RNA-guided, RNA-targeting CRISPR enzyme, commonly referred to as C2c2. This enzyme is a member of Type VI CRISPR family containing a single protein effector with two HEPN domains.  The Cas13a exhibits two RNase activities. The first activity, a cis- sequence-specific RNA guided cleavage, activates the secondary RNase activity of the enzyme, a non-specific trans- ribonuclease activity. This Cas13a protein can be applied to detect single RNA molecules with proven high specificity.


Recently, Cas13a has been leveraged as a diagnostic nucleic acid detection tool. Using a combination of the Cas13a cis- and trans- RNase activity and a non-target RNA tagged with a cleavage reporter ((fluorescence), the Cas13 system provides a route for reporting of the presence of a specific single RNA sequence in a sample with high specificity.
The GenScript Cas13a contains a His-tag at the C-terminal.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Description GenCRISPR™ Cas13a (C2c2) Nuclease is an RNA-guided RNA endonuclease from Leptotrichia wadei. This recombinant nuclease is purified from E. coli expression and contains C-terminal His Tag. Cas13a (previously known as C2c2) belongs to the Class 2 Type VI CRISPR-Cas system and contains a single protein effector with two HEPN domains. Cas13a exhibits two distinct ribonuclease activities. Cas13a possesses sequence-specific RNA cleavage activity in vitro and in vivo. And after specific cleavage, Cas13a activates collateral cleavage activity towards adjacent non-specific ssRNA sequences. The two distinct ribonuclease activities of Cas13a enable guide-RNA processing and RNA detection. The RNA detection empowered with Cas13a-crRNA complex and quenched fluorescent ssRNA as reporter has been reported to be versatile, rapid and high-specific.  

crRNA-dependent RNA cleavage

Source Recombinant Cas13a expressed by E.coli
Species Leptotrichia wadei
Tag C-terminal His Tag
Apparent Molecular Weight ~140 kDa, on SDS-PAGE under non-reducing conditions
Concentration Please refer to the COA for the specific lot.
Storage Buffer 10 mM Tris-HCl, 300 mM NaCl, 1.0 mM DTT, 50% glycerol, pH 8.0. 
Storage & Stability Store at -20 °C for up to 12 months from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C!
Accession# U2PWF1

Appearance Clear, colorless liquid
Purity ≥ 95% by SDS-PAGE
Activity ≥ 85% by in vitro digestion of ssRNA
Residual DNase Undetectable
Residual RNase Undetectable
Endotoxin Level < 0.2 EU/μg

References 1.       Abudayyeh, Omar O., et al. "C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector." Science 353.6299 (2016).
2.       Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a/C2c2." Science 356.6336 (2017): 438-442.
3.       Gootenberg, Jonathan S., et al. "Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6." Science 360.6387 (2018): 439-444.
4.       Iwasaki, Roman S., and Robert T. Batey. "SPRINT: a Cas13a-based platform for detection of small molecules." Nucleic acids research 48.17 (2020): e101-e101.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.