GenCRISPR™ Ultra ErCas12a-3NLS, His-tag

GenCRISPR™ Ultra ErCas12a-3NLS, His-tag, is a revolutionary CRISPR-associated nuclease that delivers highefficiency editing across challenging systems, including plant cells and hard-to-transfect primary T cells. Unlike traditional editors, GenCRISPR™ Ultra ErCas12a-3NLS, His-tag, operates with minimal off-target effects while maintaining robust on-target activity – even in notoriously difficult-to-edit genomes. For plant biology, broad PAM compatibility and high editing efficiency of GenCRISPR™ Ultra ErCas12a-3NLS, His-tag, enable seamless gene knockouts, insertions, and multiplexed modifications in protoplasts, callus, and regenerated plants. Its low toxicity and high specificity reduce screening workloads, accelerating trait development. In primary T cell engineering, GenCRISPR™ Ultra ErCas12a-3NLS, His-tag, outperforms conventional nucleases by achieving high knock-in efficiency without compromising cell viability.

价格	￥1747 在线订购包邮(港澳台除外)
产品编号	RC00012-0.1
规格	0.1 mg 0.5 mg 1 mg 5 mg 大单询价
数量
参考货期


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应用指导产品属性质控参数图例

Description

GenCRISPR™ Ultra ErCas12a-3NLS, His-tag, is a revolutionary CRISPR-associated nuclease that delivers high efficiency editing across challenging systems, including plant cells and hard-to-transfect primary T cells. Unlike traditional editors, GenCRISPR™ Ultra ErCas12a-3NLS, His-tag, operates with minimal off-target effects while maintaining robust on-target activity – even in notoriously difficult-to-edit genomes.

For plant biology, broad PAM compatibility and high editing efficiency of GenCRISPR™ Ultra ErCas12a-3NLS, His-tag, enable seamless gene knockouts, insertions, and multiplexed modifications in protoplasts, callus, and regenerated plants. Its low toxicity and high specificity reduce screening workloads, accelerating trait development. In primary T cell engineering, GenCRISPR™ Ultra ErCas12a-3NLS, His-tag, outperforms conventional nucleases by achieving high knock-in efficiency without compromising cell viability.

Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

应用指导

crRNA-dependent DNA cleavage

产品属性

Source	Recombinant ErCas12a with three optimized C-terminal NLS expressed by E. coli
Species	Eubacterium rectale
PAM	YTTV
Tag	N-terminal His-tag
Theoretical Molecular Weight	~156 kDa
Concentration	10 mg/ml
Storage Buffer	50 mM Sodium phosphate, 300 mM NaCl, 0.1 mM EDTA, pH 7.5, 20% (v/v) glycerol,1 mM DTT
Storage & Stability	Store at -70 ± 10 °C for 12 months from the date of manufacture. Can withstand 5 cycles of repeated freeze-thaw (-70 ± 10 °C ~ 25 ± 3 °C); but please avoid additional cycles.

质控参数

Appearance	Clear and colorless solution
Purity	≥ 95% as analyzed by SEC-HPLC ≥ 90% as analyzed by SDS-PAGE
Concentration	10 mg/mL ± 10%
Bioactivity	≥ 90%
Residual DNase	< LOQ by In-house residual RNase detection assay
Residual RNase	< LOQ by In-house residual RNase detection assay
Endotoxin Level	≤ 10 EU/mg as analyzed by LAL Kinetic-Chromogenic Assay

图例