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| E00049 | |
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Hot Start Taq DNA Polymerase is a recombinant, thermostable Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the polymerase activity prior to the initial DNA denaturation step of PCR. When the temperature of the PCR reaction mix reaches 95°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored. |
| Key Features |
Enhanced specificity of amplification Reduced primer-dimer formation Increased yield of the PCR products Increased convenience of reactions set up at room temperature (useful in applications such as colony PCR) |
| Storage & Stability | Please store at -20°C. |
| Hot Start Taq DNA Polymerase is used for PCR amplification with enhanced specificity. |
The primer extension assay to detect the blocking activity of Taq Antibody.
To test the blocking activity of Hot Start Taq antibody, a primer extension assay was done as follows. A pair of primers was designed with a 14 bp overlap. One is 24 bp and the other one is 41 bp. The primers were annealed and incubated with Taq DNA polymerase (lane 1-2) or Hot Start Taq DNA polymerase (lane 3-4) for 0.5 h at 65℃. The extension result was separated on an Urea PAGE gel. No primer dimers were observed with Hot Start Taq polymerase. »
The PCR specificity detection of Hot Start Taq DNA polymerase.
To test the specificity of amplification, a 500 bp long HPRT fragment was amplified from human genomic DNA using Hot Start Taq DNA polymerase (lanes 1-2) and Taq DNA polymerase (lane 3-4). No non-specific bands were observed in the amplification reaction with the Hot Start Taq DNA polymerase. »
