目录产品 » GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse
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GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse

This product is specific for Human CD3
询价
GMPA02199

产品概述
Specificity This product is specific for Human CD3
Host Species Mouse
Immunogen Recombinant Human CD3
Conjugate Unconjugated
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应用指导
ex vivo cell culture process; Flow cytometry
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产品属性
Form Liquid
Storage Buffer Supplied in 10 mM Sodium Citrate, 150 mM NaCl, 0.02 mg/ml Polysorbate 80, pH 5.0
Concentration 5 mg/ml
Storage Instructions The product remains stable at -70 ± 10 °C. Avoid repeated freeze-thaw cycles. Please refer to the COA for specific expiry date.
Isotype Mouse IgG2a
Clonality Monoclonal
Clone ID OKT3
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图例
GMP Anti-Human CD3 Antibody (OKT3), MAb, Mouse

GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. GMPA02199) was used for primary T cell activation together with anti-Human CD28 antibody. The percentage of activated T cells was measured by flow cytometry. Data showed that GenScript GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse outperformed the competing products in activating primary T cells. »

GMP Anti-Human CD3 Antibody (OKT3), MAb, Mouse

Jurkat/IL-2-Luc Promoter cells were co-stimulated with a serial of concentrations of GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. GMPA02199) and GMP Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. GMPA02213) for 6 h. Receptor-mediated signaling induced luminescence via the activation of the IL-2 pathway. »

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质控参数
外观 Clear, colorless liquid
pH 5.0 ± 0.5
Concentration 5.0 ± 0.5 mg/ml tested by A280 nm
Purity ≥ 95.0% as analyzed by SEC-HPLC
Sequence The coverage of the light chain and heavy chain sequence was 100% consistent with the theoretical amino acid sequence by mass spectrometry (MS)
Residual HCP ≤ 100.0 ng/mg by ELISA method
Residual HCD ≤ 10.0 pg/mg by quantitative PCR method
Residual Protein A ≤ 10.0 ng/mg by ELISA method
Endotoxin Level < 0.1 EU/mg by gel clotting method
Sterility No growth
Reactivity Qualified
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技术背景
Target Background CD3 is a member of the immunoglobulin superfamily, expressed on T cells. It is composed of four distinct chains (ε, γ, δ, ζ). CD3 antigen can form a complex with the T cell receptor (TCR) to generate an activation signal in T cells, which plays an important role in the transduction of TCR signal and the activation of both cytotoxic T cells (CD8 + naïve T cells) and T helper cells (CD4+ naïve T cells).
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For laboratory research and ex vivo cell isolation and culture applications. Direct human use, including taking orally and injection and clinical use are forbidden.