| Specificity |
This product is specific to PHD3 |
| Host Species |
Rabbit |
| Immunogen |
A synthesized peptide derived from human PHD3 |
| Species Reactivity |
Human; Mouse; Rat |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Western Blot |
1:1000-1:2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF) |
1:50-1:200 |
| Immunoprecipitation (IP) |
1:20-1:50 |
|
| Form |
Liquid |
| Storage Buffer |
Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Storage Instructions |
Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification |
Affinity-chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Target Background |
Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as PKM, TELO2, ATF4 and HIF1A.Target proteins are preferentially recognized via a LXXLAP motif. Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. |
| Synonyms |
Egl nine homolog 3; EGLN3; Factor responsive smooth muscle protein; HIF Prolyl Hydroxylase 3; HIFP4H3; HIFPH3; P4H3; PHD3; SM20; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
