目录产品 » Pyruvic carboxylase Antibody, mAb, Rabbit
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Pyruvic carboxylase Antibody, mAb, Rabbit

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
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A04843-50

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产品概述
Specificity This product is specific to Pyruvic carboxylase
Host Species Rabbit
Immunogen A synthesized peptide derived from human Pyruvic carboxylase
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
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应用指导
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
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产品属性
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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图例
Pyruvic Carboxylase Antibody, MAb, Rabbit

Western blot analysis of PCB expression in HepG2 cell lysate. »

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技术背景
Target Background Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Synonyms PC; PCB; Pcx; Pyruvate carboxylase;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.