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NONO Antibody, mAb, Rabbit

DNA- and RNA binding protein, involved in several nuclear processes.Binds the conventional octamer sequence in double-stranded DNA.Also binds single-stranded DNA and RNA at a site independent of the duplex site.Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ.
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A04703-50

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产品概述
Specificity This product is specific to NONO
Host Species Rabbit
Immunogen A synthesized peptide derived from human NONO
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
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应用指导
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
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产品属性
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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图例
NONO Antibody, MAb, Rabbit

Western blot analysis of NONO / p54nrb expression in (1) MOLT4 cell lysate; (2) NIH/3T3 cell lysate; (3) PC12 cell lysate. »

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技术背景
Target Background DNA- and RNA binding protein, involved in several nuclear processes.Binds the conventional octamer sequence in double-stranded DNA.Also binds single-stranded DNA and RNA at a site independent of the duplex site.Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ.
Synonyms P54; NMT55; NRB54; P54NRB;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.