| Specificity |
This product is specific to Cullin 3 |
| Host Species |
Rabbit |
| Immunogen |
A synthesized peptide derived from human Cullin 3 |
| Species Reactivity |
Human; Mouse; Rat |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Western Blot |
1:1000-1:2000 |
| Immunoprecipitation (IP) |
1:20-1:50 |
| Flow Cytometry |
1:20-1:100 |
|
| Form |
Liquid |
| Storage Buffer |
Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Storage Instructions |
Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification |
Affinity-chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Target Background |
Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. BCR complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. |
| Synonyms |
cul3; Cullin3; PHA2E; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
