目录产品 » p-POLR2A (S5) Antibody, mAb, Rabbit
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p-POLR2A (S5) Antibody, mAb, Rabbit

During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
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A03658-50

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产品概述
Specificity This product is specific to p-POLR2A (S5)
Host Species Rabbit
Immunogen A synthesized peptide derived from human POLR2A around the phosphorylation site of S5
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated
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应用指导
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50
Flow Cytometry 1:20-1:100
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产品属性
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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图例
P-POLR2A (S5) Antibody, MAb, Rabbit

Western blot analysis of Phospho-POLR2A (S5) expression in (1) HeLa cell lysate treated with Lambda Phosphatase; (2) HeLa cell lysate. »

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技术背景
Target Background During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.
Synonyms POLR2A; POLR2; RNA polymerase II CTD repeat YSPTSPS;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.