目录产品 » SMAD1 Antibody, mAb, Rabbit
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SMAD1 Antibody, mAb, Rabbit

Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis (PubMed:9335504).Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor.
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A03254-50

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产品概述
Specificity This product is specific to SMAD1
Host Species Rabbit
Immunogen A synthesized peptide derived from human SMAD1
Species Reactivity Human
Conjugate Unconjugated
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应用指导
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Flow Cytometry 1:20-1:100
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产品属性
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
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图例
SMAD1 Antibody, MAb, Rabbit

Western blot analysis of Smad1 expression in 293T cell lysate. »

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技术背景
Target Background Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis (PubMed:9335504).Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor.
Synonyms MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; BSP-1; SMAD1; BSP1; MADH1; MADR1;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.