| Specificity |
This product is specific for double-stranded RNA (dsRNA) with the length of the helix greater than or equal to 40 bp. The dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. |
| Host Species |
Mouse |
| Immunogen |
Double-stranded RNA |
| Conjugate |
Biotin |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Dot Blot |
1:200-1:500 dilution |
| Sandwich ELISA |
1:100-1:250 dilution |
|
| Form |
Liquid |
| Storage Buffer |
Supplied in PBS (pH 7.2), containing 10 mg/ml BSA, 0.02% sodium azide and 50% glycerol. |
| Concentration |
0.5 mg/ml |
| Storage Instructions |
Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG2a, κ |
| Clonality |
Monoclonal |
| Clone ID |
J2 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
![Double-stranded RNA (dsRNA) Antibody (J2) [Biotin], MAb, Mouse](/product/documents/down?doc_name=A02356_Dot Blot.jpg&file=scm_files/productFile_notes/2025/03/28/c610688b-349b-44e7-a6b6-4e2868a09693.jpg "Double-stranded RNA (dsRNA) Antibody (J2) [Biotin], MAb, Mouse")
The results showed that J2 antibody (GenScript, A02181), J2 antibody [Biotin] (GenScript, A02356) and J2 antibody [HRP] (GenScript, A02357) can be used for dsRNA Dot Blot assay development.
| Target Background |
Double-stranded RNA (dsRNA) forms in nucleic acid strands containing complementary sequences. In gene therapy, minimization and removal of double-stranded RNA (dsRNA) is important for mRNA in vitro transcription (IVT) processes. Anti-dsRNA monoclonal antibody J2 is a useful tool in dsRNA detection. J2 antibody also can be used to detect dsRNA intermediates of Hepatitis C virus, Dengue virus, rhinovirus and Chikungunya virus. Since it was first published in 1991, J2 antibody has been broadly widely used to detect viral dsRNA in animals and plants in more than 200 scientific publications. The original J2 antibody was prepared by immunization of mice with L-dsRNA. |
| Synonyms |
Double-stranded RNA, dsRNA |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
