| ￥1580 | |
| A02342-100 | |
|
|
|
|
|
|
|
|
|
| Specificity | MonoRab™ Anti-Strep Tag II Antibody, mAb, Rabbit recognizes C-terminal, N-terminal and internal Strep Tag II (WSHPQFEK) fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes Twin-Strep-tag II (WSHPQFEKGGGSGGGSGGGSWSHPQFEK) fusion proteins. |
| Host Species | Rabbit |
| Immunogen | WSHPQFEK peptide conjugated to KLH |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||||
|
|||||||||
| Form | Liquid |
| Storage Buffer | Supplied in PBS (pH 7.2), containing 10 mg/ml BSA, 0.03% Proclin 300 and 50% glycerol. |
| Storage Instructions | Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification | Protein A affinity column |
| Isotype | Rabbit IgG, κ |
| Clonality | N/A |
| Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
A comparative detection of E.coli, Yeast, CHO-S, and HEK-293 whole cell lysate without Strep Tag II Protein by Western blot was performed using MonoRab™ Anti-Strep Tag II Antibody, mAb, Rabbit (GenScript, A02342) and Strep Tag II Antibody (Competitor A), both at a 1:1000 dilution.
C-terminal, N-terminal Strep tag II fusion proteins served as positive controls. »
A comparative detection of N-terminal Strep tag II fusion proteins by Western blot was performed using MonoRab™ Anti-Strep Tag II Antibody, mAb, Rabbit (GenScript, A02342) and Strep Tag II Antibody (Competitor A), both at a 1:1000 dilution.
Each antibody was used to detect the same fusion proteon sample containing a N-terminal Strep tag II. »
A comparative detection of C-terminal Strep tag II fusion proteins by Western blot was performed using MonoRab™ Anti-Strep Tag II Antibody, mAb, Rabbit (GenScript, A02342) and Strep Tag II Antibody (Competitor A), both at a 1:1000 dilution.
»
A comparative detection of twin-Strep tag II fusion proteins by Western blot was performed using MonoRab™ Anti-Strep Tag II Antibody, mAb, Rabbit (GenScript, A02342) and Strep Tag II Antibody (Competitor A), both at a 1:1000 dilution.
Each antibody was used to detect the same fusion protein sample containing a twin-Strep tag II. »
BLI (Biolayer interferometry) binding affinity measurements of MonoRab™ Anti-Strep Tag II Antibody, mAb, Rabbit (GenScript, A02342) to Strep tag II fusion proteins.
A02342 was captured using a protein A probe and subsequently bound to a Strep tag II fusion protein.The dissociation constants (KD) for the N-terminal Strep tag II, C-terminal Strep tag II, and twin-Strep tag II fusion proteins are 2.04 nM, 160 pM, and ≤1 pM, respectively. This demonstrates a higher affinity compared to the affinity of Strep-Tactin XT for Strep tag II (~ 27 nM) and twin-Strep tag II (~ 62 pM). »
Anti-Strep Tag II Antibody binds to Strep tag II fusion proteins in ELISA assay.
C-terminal, N-terminal, Internal Strep tag II fusion proteins, or twin-Strep tag II fusion protein were coated at a concentration of 1 μg/mL. Subsequently, MonoRab™ Anti-Strep Tag II Antibody, mAb, Rabbit (GenScript, A02342) was serially diluted starting from 1 μg/mL and added. Detection was carried out using Mouse Anti-Rabbit IgG Fc Antibody (14H9H10) [HRP], mAb (GenScript, A01856) diluted at 1:20,000. »
| Target Background | The Strep-tag II consists of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys), whereas the Twin-Strep-tag includes this motif two times in series connected by a linker and is accordingly composed of 28 amino acids. Strep-tag II are commonly used as an affinity tag in protein purification and detection and are known for its high binding affinity to streptavidin mutants. The strep-tag II has been widely used in various molecular biology and protein research applications due to its specificity and versatility. |
| Synonyms | Strep-tag II; Strep tag; Twin-Strep-tag |
For research use only. Not intended for human and animal therapeutic or diagnostic use.
