MonoRab™ Rabbit Anti-scFv Cocktail (Min X) [iFluor 647]

This product is specific for scFvs in various species (humanized, mouse), various orders (VH-linker-VL, VL-linker-VH) and various forms of scFv-based bispecific antibodies(DART, BiTE, TandAbs, IgG(H)-scFv2, scFv-Fab-Fc, etc). This product exhibits minimal cross-reactivity with human, mouse, goat, and rabbit immunoglobulins.

价格	￥3450 在线订购包邮(港澳台除外)
产品编号	A02305-200
规格	200 μl 大单询价
数量
参考货期


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产品概述应用指导产品属性图例技术背景有限使用许可与使用限制

产品概述

Specificity	This product is specific for scFvs in various species (humanized, mouse), various orders (VH-linker-VL, VL-linker-VH) and various forms of scFv-based bispecific antibodies(DART, BiTE, TandAbs, IgG(H)-scFv2, scFv-Fab-Fc, etc). This product exhibits minimal cross-reactivity with human, mouse, goat, and rabbit immunoglobulins.
Host Species	Rabbit
Immunogen	This information is confidential and belongs to GenScript.
Conjugate	iFluor 647

应用指导

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Flow Cytometry	1 µg/ml-10 µg/ml

产品属性

Form	Liquid
Storage Buffer	Supplied in PBS (pH 7.2), containing 10 mg/ml BSA, 0.02% sodium azide and 50% glycerol.
Concentration	0.5 mg/ml
Storage Instructions	Store at -20°C and it should be protected from prolonged exposure to light. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification	Protein A affinity column
Isotype	Rabbit IgG, κ
Clonality	Monoclonal antibody cocktail
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

图例

Flow cytometric analysis of untransduced T cells or CAR-transduced T cells using MonoRab™ Rabbit Anti-scFv Cocktail (Min X) [iFluor 647] (GenScript, A02305).

The results show that MonoRab™ Rabbit Anti-scFv Cocktail (Min X) [iFluor 647] can achieve good cell clustering for CAR-transduced T cells and show minimal non-specificity for untransduced T cells.

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Flow cytometric analysis of Raji cells using anti-CD19 scFv and MonoRab™ Rabbit Anti-scFv Cocktail (Min X) [iFluor 647] (GenScript, A02305) (Red peak) compared to A02305 alone (green).

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技术背景

Target Background

A single chain fragment variable (scFv) is a type of recombinant antibody. It is approximately 25 kDa and consists of the variable regions of the heavy (VH) and light (VL) chains of an antibody, which are connected by a flexible peptide linker. scFvs have several advantages, including their small molecular weight, strong penetration, and high specificity. They play crucial roles in targeted therapy, imaging diagnosis, and biological detection. Importantly, in the field of cell therapy, scFvs can act as the antigen recognition domain of CAR-T cells and determine the targeting ability of these cells.

Synonyms

scFv; Single-chain fragment variable; Single chain antibody; Single-chain variable region fragment; Single-chain fv

有限使用许可与使用限制

有限使用许可与使用限制

This Product is provided under a limited, non-exclusive, non-transferable, and non-sublicensable end-user license strictly limited to the permitted uses set forth in GenScript’s official documentation, quotations, or written agreements. Any use beyond such scope requires GenScript’s prior written authorization or license.
Except as expressly authorized in writing by GenScript, Customer shall not, and shall not permit, procure, enable, or assist any third party to: (a) commercialize or otherwise exploit the Product, including by reselling, distributing, transferring, sublicensing, or otherwise making the Product (including any product incorporating the Product) available to any third party; (b) copy, reproduce, clone, reverse clone, sequence, analyze, characterize, deconstruct, reconstruct, reverse engineer, or otherwise attempt to discover the composition, sequence, structure, binding characteristics, epitope recognition characteristics, or other proprietary features of the Product; (c) modify, derivatize, humanize, optimize, mutate, design around, or generate any variants, derivatives, fragments, substitutes, or functional equivalents of the Product; or (d) use the Product, any information derived therefrom, or any computational, AI-enabled, or sequence-based design method to develop, manufacture, offer for sale, sell, or commercialize any product or service competitive with the Product or GenScript’s products or services.
All intellectual property rights and all rights not expressly granted herein are reserved by GenScript. No rights are granted by implication, estoppel, or otherwise.

For research use only. Not intended for human and animal therapeutic or diagnostic use.