目录产品 » MonoRab™ Rabbit Anti-scFv Cocktail [iFluor 488]
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MonoRab™ Rabbit Anti-scFv Cocktail [iFluor 488]

This product is specific for scFvs in different species (humanized, mouse) and different orders (VH-linker-VL, VL-linker-VH). It can also recognize various forms of scFv-based bispecific antibodies, such as DART, BITE, TandAbs, IgG(H)-scFv2, scFv-Fab-Fc, etc.
￥3150 在线订购包邮(港澳台除外)
A02286-200

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产品概述
Specificity This product is specific for scFvs in different species (humanized, mouse) and different orders (VH-linker-VL, VL-linker-VH). It can also recognize various forms of scFv-based bispecific antibodies, such as DART, BiTE, TandAbs, IgG(H)-scFv2, scFv-Fab-Fc, etc.
Host Species Rabbit
Immunogen This information is confidential and belongs to GenScript.
Conjugate iFluor 488
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应用指导
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Flow Cytometry 1 µg/ml-10 µg/ml
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产品属性
Form Liquid
Storage Buffer Supplied in PBS (pH 7.2), containing 10 mg/ml BSA, 0.02% sodium azide and 50% glycerol.
Concentration 0.5 mg/ml
Storage Instructions Store at -20°C and it should be protected from prolonged exposure to light. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Protein A affinity column
Isotype Rabbit IgG, κ
Clonality Monoclonal antibody cocktail
Note GenScript can customize this product per customer's request including product size, buffer components, etc.
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图例
MonoRab™ Rabbit Anti-scFv Cocktail [iFluor 488]

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MonoRab™ Rabbit Anti-scFv Cocktail [iFluor 488]

Flow cytometric analysis of Raji cells using anti-CD20 scFv and MonoRab™ Rabbit Anti-scFv Cocktail [iFluor 488] (GenScript, A02286) (Blue peak) compared to A02286 alone (green) or unstained cells (red peak).
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技术背景
Target Background A single chain fragment variable (scFv) is a type of recombinant antibody. It is approximately 25 kDa and consists of the variable regions of the heavy (VH) and light (VL) chains of an antibody, which are connected by a flexible peptide linker. scFvs have several advantages, including their small molecular weight, strong penetration, and high specificity. They play crucial roles in targeted therapy, imaging diagnosis, and biological detection. Importantly, in the field of cell therapy, scFvs can act as the antigen recognition domain of CAR-T cells and determine the targeting ability of these cells.
Synonyms scFv; Single-chain fragment variable; Nanobody; Single chain antibody; VH and VL of an antibody; Single-chain variable region fragment; Single-chain fv; VH-linker-VL; VL-linker-VH
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For research use only. Not intended for human and animal therapeutic or diagnostic use.