BLI (Biolayer interferometry) binding affinity measurements of MonoRab™ Anti-Lecanemab Antibody (4F2), mAb, Rabbit (GenScript, A02271) to Lecanemab.
Lecanemab captured on HFC (Anti-Human IgG Fc) Probes can bind MonoRab™ Anti-Lecanemab Antibody (4F2), mAb, Rabbit with a dissociation constant (KD) of less than 1 pM.

Anti-Lecanemab Antibody (44G1) or (4F2) inhibits the binding of Lecanemab to β-Amyloid (1-42). Coating antigen: β-Amyloid (1-42), human (GenScript, RP10017) 1 µg/ml.
Lecanemab final concentration: 10 µg/ml.
Anti-Lecanemab antibody dilutions start from 10 μg/ml.
MonoRab™ Anti-Lecanemab Antibody (44G1), mAb, Rabbit (GenScript, A02270) and MonoRab™ Anti-Lecanemab Antibody (4F2), mAb, Rabbit (GenScript, A02271) demonstrated inhibitory properties.

Standard Curve of Lecanemab Sandwich ELISA by A02270 (44G1) and A02271 (4F2)
In this Sandwich ELISA assay, MonoRab™ Anti-Lecanemab Antibody (44G1), mAb, Rabbit (GenScript, A02270) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Lecanemab Antibody (4F2), mAb, Rabbit (GenScript, A02271) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL. GenScript can provide customized conjugation service for this product per customer's request.
In this assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.99971. The typicaldynamic range of the assay is 0.31 - 20 ng/mL and its detection limit is 0.31 ng/mL.

MRD Analysis of the Detection of Lecanemab in Biological Matrix.
The MRD is the minimum dilution necessary for the detection of Lecanemab in biological matrix with least interference. Serum samples from cynomolgus monkey were serially diluted to determine the MRD of this assay, and the test result suggested that MRD was as 1:10.
In this ELISA assay, MonoRab™ Anti-Lecanemab Antibody (44G1), mAb, Rabbit (GenScript, A02270) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Lecanemab Antibody (4F2), mAb, Rabbit (GenScript, A02271) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL.

Specificity analysis of the detection of Lecanemab.
Lecanemab samples at 8 concentrations were spiked with 200 ng/mL of human IgG1. The test result demonstrated that the high concentration of human IgG1 did not interfere with the detection of Lecanemab.
In this ELISA assay, MonoRab™ Anti-Lecanemab Antibody (44G1), mAb, Rabbit (GenScript, A02270) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Lecanemab Antibody (4F2), mAb, Rabbit (GenScript, A02271) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL.