| ￥1600 在线订购包邮(港澳台除外) | |
| A02225-40 | |
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| Specificity | The product is specific for MMAF and MMAF structurally similar molecules. |
| Host Species | Mouse |
| Immunogen | MMAF-KLH |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||||
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| Form | Liquid |
| Storage Buffer | Supplied in PBS, pH 7.4, containing 0.02% ProClin300. |
| Concentration | 0.5 mg/mL |
| Storage Instructions | The product remains stable for 1 year at -20℃ from the date of receipt. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG2b, κ |
| Clonality | Monoclonal |
| Clone ID | 11B8A6 |
| Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
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ELISA binding of MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) with MMAF, MMAE, DXd and DM1 conjugated antibody respectively.
The coating antibody was MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) used at 2 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including MMAF, MMAE, DXd and DM1 conjugated antibodies used at 1 μg/mL concentration. The secondary antibody was Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) used at 4 μg/ml concentration.
MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) is specific to MMAF and has no cross-reactivity with MMAE, DXd, and DM1. »
Standard curve of MMAF Competitive ELISA.
The competitive ELISA assay is developed by using free MMAF block the binding of MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) to HRP conjugated MMAF.
Capture antibody: MMAF Antibody (11B8), mAb, Mouse, 2 μg/ml
Detection reagent: HRP conjugated MMAF, 10 μg/ml
Free MMAF dilution start from 2 μg/ml. »
Standard curve of MMAF conjugated Pembrolizumab Sandwich ELISA by anti-Human IgG Fc antibody and A02225.
The Sandwich ELISA assay is developed by using MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) and Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) as the capture and detection antibodies, respectively.
In this ELISA assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.997.
Capture antibody: MMAF Antibody (11B8), mAb, Mouse, 2 μg/ml
Detection antibody: Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb, 4 μg/ml »
Standard curve of MMAF conjugated Pembrolizumab Sandwich ELISA by anti-idiotype antibody and A02225.
The Sandwich ELISA assay is developed by using MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) and MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], mAb, Rabbit (GenScript, A01853) as the capture and detection antibodies, respectively.
In this ELISA assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.99974.
Capture antibody: MMAF Antibody (11B8), mAb, Mouse, 2 μg/ml
Detection antibody: MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], mAb, Rabbit, 0.05 μg/ml »
| Target Background | Payloads are the important components of antibody-drug conjugates (ADCs). The most commonly used ADC payloads are MMAE, MMAF, DM1, and DXd. ADC pharmacokinetic (PK) studies include free payload analysis, conjugated antibody analysis, and total antibody analysis via ligand-binding assay (LBA) and LC-MS/MS assay. The anti-payload antibody is a useful reagent in PK assay for determining conjugated antibodies. It can be used as a capture antibody to determine the conjugated antibody in LBA assay or through immunocapture to enrich the ADC sample in LC-MS/MS assay. The MMAF, also known as Monomethylauristatin F, is a potent tubulin polymerization inhibitor. It is used as an antimitotic drug in the form of antibody drug conjugates (ADCs). |
| Synonyms | Monomethylauristatin F, Monomethyl Auristatin F, 745017-94-1 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
