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THE™ V5 Tag Antibody, mAb, Mouse

THE™ V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
¥1800
A01724

Specificity THE™ V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
Host Species Mouse
Immunogen V5 tag peptide conjugated to KLH
Conjugate Unconjugated
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.1 µg/ml
Western Blot 0.2-1 µg/ml
Immunoprecipitation (IP) 1-5 µg/ml
Flow Cytometry 1-3 µg for 1 x 106 cells
Immunocytochemistry/Immunofluorescence (ICC/IF) 1-3 µg/ml
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Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, ti can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2a,κ
Clonality Monoclonal
Clone ID 4C12E11
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THE™ V5 Tag Antibody, MAb, Mouse

Sensitivity comparison of purified internal V5 tagged fusion proteins with V5 Tag Antibody, mAb, Mouse from GenScript, (A01724, 0.2 µg/ml) and Invitrogen (R960-25, 0.2 µg/ml) by Western blot. The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG »

THE™ V5 Tag Antibody, MAb, Mouse

Immunocytochemistry/Immunofluenrescence analysis of non-transfected CHO cells (a) and V5 fusion gene transfeced CHO cells (b) using THETM V5 Antibody, mAb, Mouse (GenScript, A01724). The signal was developed with FITC conjugated Goat Anti-Mouse IgG. »

THE™ V5 Tag Antibody, MAb, Mouse

Comparison sensitivity of purified N-terminal V5 tagged fusion proteins with V5 Tag Antibody, mAb, Mouse from GenScript (A01724, 0.2 µg/ml) and Invitrogen ( R960-25, 0.2 µg/ml) by Western blot. The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG »

THE™ V5 Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected or V5 fusion gene transfected CHO cells using
THETM V5 Tag Antibody, mAb, Mouse (GenScript, A01724) (black and red respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG. »

THE™ V5 Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected CHO cells (a) and V5 fusion gene transfeced CHO cells (b) using THETM V5 Antibody, mAb, Mouse (GenScript, A01724). The signal was developed with FITC conjugated Goat Anti-Mouse IgG. »

THE™ V5 Tag Antibody, MAb, Mouse

Western blot analysis of immunoprecipitates from V5-tagged protein transfected HEK293 cell lysates using THETM V5 Antibody, mAb, Mouse (A01724, 1 µg/ml).
Lane 1: V5-tagged protein transfected HEK293 cell lysate as control.
Lane 2-3: Immunoprecipitates of V5-tagged protein transfected and non-transfected HEK293 cell lysate with V5 Antibody, mAb, Mouse.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG. »

THE™ V5 Tag Antibody, MAb, Mouse

Sensitivity comparison of purified internal V5 tagged fusion proteins with V5 Tag Antibody, mAb, Mouse from GenScript, (A01724, 0.2 µg/ml) and Invitrogen (R960-25, 0.2 µg/ml) by Western blot. The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG. The assay was performed with internal V5 tagged fusion protein. »

THE™ V5 Tag Antibody, MAb, Mouse

Superior specificity assay of transfected cell lysates using Western blot with THE™ V5 Antibody, mAb, Mouse (GenScript, A01724).
Lane 1: V5-tagged protein transfected CHO cell lysate.
Lane 2: Non-transfected CHO cell lysate.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG. »

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Target Background The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.THE™ V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.
Synonyms THE™ V5 Tag Antibody, mAb, Mouse;
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Hitoshi Matakatsu , et al. Dachsous and Fat coordinately repress the Dachs–Dlish–Approximated complex to control growth. J Cell Biol. (2023-12)
Brian G Peterson, et al. Deep mutational scanning highlights a new role for cytosolic regions in Hrd1 function. biorxiv. (2023-04)
Jacob A Gibson, et al. Sequestration of a dual function DNA-binding protein by Vibrio cholerae CRP. Proc Natl Acad Sci U S A. (2022-11)
Hannah M Maul-Newby, et al. A model for DHX15 mediated disassembly of A-complex spliceosomes. RNA. (2022-01)
Eva Pauwels, et al. Reduced DNAJC3 Expression Affects Protein Translocation across the ER Membrane and Attenuates the Down-Modulating Effect of the Translocation Inhibitor Cyclotriazadisulfonamide. Int J Mol Sci. (2022-01)
Zhenke Yang, et al. A malaria parasite phospholipid flippase safeguards midgut traversal of ookinetes for mosquito transmission. Sci Adv. (2021-07)
Shan Wang, et al. Mithramycin suppresses DNA damage repair via targeting androgen receptor in prostate cancer. Cancer Lett. (2020-05-01)
Yenerall P, et al. RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer. Cell chemical biology. (2020-01)
Anqun Chen, et al. Soluble RARRES1 induces podocyte apoptosis to promote glomerular disease progression. J Clin Invest. (2020)
Wei C, et al. SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and Affects Albuminuria in Allografts. J Am Soc Nephrol. (2018)
Lee Seung Woo, et al. The synergistic effect of combination temozolomide and chloroquine treatment is dependent on autophagy formation and p53 status in glioma cells. Cancer Lett. (2015)
Kim HS, et al. Inositol Polyphosphate-5-Phosphatase F (INPP5F) inhibits STAT3 activity and suppresses gliomas tumorigenicity. Sci Rep. (2014-12)
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