THE™ GFP Antibody, pAb, Rabbit

GenScript THE™ GFP Antibody, pAb, Rabbit specifically reacts with fusion proteins containing GFP epitope tag. This antibody can recognize all variants of GFP protein such as eGFP, AcGFP1 and cGFP. It slightly recognizes a variety of variants of RFP protein such as DsRed, DsRed2, mOrange, mCherry and tdTomato.

价格	￥2600 在线订购包邮(港澳台除外)
产品编号	A01704-100
规格	100 μg 大单询价
数量
参考货期


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产品概述应用指导产品属性技术背景

产品概述

Specificity	GenScript THE™ GFP Antibody, pAb, Rabbit specifically reacts with fusion proteins containing GFP epitope tag. This antibody can recognize all variants of GFP protein such as eGFP, AcGFP1 and cGFP. It slightly recognizes a variety of variants of RFP protein such as DsRed, DsRed2, mOrange, mCherry and tdTomato.
Host Species	Rabbit
Immunogen	purified recombinant full-length GFP protein
Conjugate	Unconjugated

应用指导

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Western Blot	0.2-1 µg/ml
Immunoprecipitation (IP)	1-5 µg/ml
Flow Cytometry	1-3 µg for 1 x 10⁶ cells
Immunocytochemistry/Immunofluorescence (ICC/IF)	1-3 µg/ml

产品属性

Form	Lyophilized
Storage Buffer	lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions	The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles.
Purification	Affinity chromatography
Isotype	Rabbit IgG
Clonality	Polyclonal
Clone ID	Not applicable
Note	Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap.

技术背景

Target Background

Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y.GenScript THE™ GFP Antibody, pAb, Rabbit is developed in rabbit using purified recombinant full-length GFP protein.

Synonyms

Green Fluorescent Protein antibody;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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