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目录产品 » 抗体产品 » 标签抗体 » THE™ DYKDDDDK Tag Antibody, mAb, Mouse

THE™ DYKDDDDK Tag Antibody, mAb, Mouse

Specificity THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins.
Host Species Mouse
Immunogen A synthetic peptide (DYKDDDDK) coupled to KLH
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Immunoprecipitation 1 µg/ml
Immunofluorescent staining 1 µg/ml
Flow cytometry 1 µg/ml
Other applications User-optimized

Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2b,κ
Clonality Monoclonal
Clone ID 5A8E5
Note GenScript can offer this product per customer's reuqest including product size, buffer components,etc.

Target Background The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.It has been used extensively as a general epitope tag in expression vectors.THE™ DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.
Synonyms Mouse anti DYKDDDDK-tag mAb;Mouse anti flag-tag mAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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产品编号: A00187
规格
价格
308 A00187-100 100 μg ¥1000