THE™ DYKDDDDK Tag Antibody, mAb, Mouse

THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK tagged fusion proteins.

价格	￥1000 在线订购包邮(港澳台除外)
产品编号	A00187-100
规格	100 μg 大单询价
数量
参考货期


咨询产品问题

产品概述应用指导产品属性技术背景

产品概述

Specificity	THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK tagged fusion proteins.
Host Species	Mouse
Immunogen	A synthetic peptide (DYKDDDDK) coupled to KLH
Conjugate	Unconjugated

应用指导

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.1-1.0 µg/ml
Immunoprecipitation (IP)	1 µg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF)	1 µg/ml
Flow Cytometry	1 µg/ml

产品属性

Form	Lyophilized
Storage Buffer	Lyophilized with PB, pH 6.5, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
Storage Instructions	The product remains stable for 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG2b,κ
Clonality	Monoclonal
Clone ID	5A8E5
Note	GenScript can offer this product per customer's request including product size, buffer components, etc.

技术背景

Target Background	The DYKDDDDK peptide is a small epitope tag which is widely used in expression vectors. The tag does not appear to interfere with the bioactivity or bio-distribution of the tagged recombinant proteins. THE™ DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins
Synonyms	Mouse anti DYKDDDDK-tag mAb; Mouse anti flag-tag mAb

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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