THE™ DYKDDDDK Tag Antibody, mAb, Mouse

THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK tagged fusion proteins.

价格	￥1000
产品编号	A00187-100
规格	100 μg 大单询价
数量

产品概述应用指导产品属性技术背景

产品概述

Specificity	THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK tagged fusion proteins.
Host Species	Mouse
Immunogen	A synthetic peptide (DYKDDDDK) coupled to KLH
Conjugate	Unconjugated

应用指导

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.1-1.0 µg/ml
Immunoprecipitation (IP)	1 µg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF)	1 µg/ml
Flow Cytometry	1 µg/ml

产品属性

Form	Lyophilized
Storage Buffer	Lyophilized with PB, pH 6.5, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
Storage Instructions	The lyophilized product remains stable for up to 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG2b,κ
Clonality	Monoclonal
Clone ID	5A8E5
Note	GenScript can offer this product per customer's request including product size, buffer components, etc.

技术背景

Target Background	The DYKDDDDK peptide is a small epitope tag which is widely used in expression vectors. The tag does not appear to interfere with the bioactivity or bio-distribution of the tagged recombinant proteins. THE™ DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins
Synonyms	Mouse anti DYKDDDDK-tag mAb; Mouse anti flag-tag mAb

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

相关文献

1. Xian Du, et al. RBM22 regulates RNA polymerase II 5' pausing, elongation rate, and termination by coordinating 7SK-P-TEFb complex and SPT5. Genome Biology . (2024-04)
2. Seungwon Jung , et al. Role of UPF1-LIN28A interaction during early differentiation of pluripotent stem cells. Nat Commun. (2024-01)
3. Chao Zheng , et al. Elevating plant immunity by translational regulation of a rice WRKY transcription factor. Plant Biotechnol J . (2023-11)
4. Salini Thulasirajah, et al. A De Novo Missense Variant in TUBG2 in a Child with Global Developmental Delay, Microcephaly, Refractory Epilepsy and Perisylvian Polymicrogyria. Genes. (2022-12)
5. Kyung-Mi Choi, et al. Defective brown adipose tissue thermogenesis and impaired glucose metabolism in mice lacking Letmd1. Cell Rep. (2021-12)
6. Sukanya Srinivasan, et al. Alpha adaptins show isoform-specific association with neurofibrillary tangles in Alzheimer's disease. Neuropathol Appl Neurobiol. (2021-11)
7. Zu Ye, et al. GRB2 enforces homology-directed repair initiation by MRE11. Sci Adv. (2021-08)
8. Yue Qi, et al. The c824C>A and c616dupA mutations in the SLC17a8 gene are associated with auditory neuropathy and lead to defective expression of VGluT3. Neuroreport. (2021-08)
9. Yuan Fang, et al. The H3K36me2 methyltransferase NSD1 modulates H3K27ac at active enhancers to safeguard gene expression. Nucleic Acids Res. (2021-06)
10. Zhang H, et al. CPEB3-mediated MTDH mRNA translational suppression restrains hepatocellular carcinoma progression. Cell death & disease. (2020-09)
11. Kim SC, et al. Nuclear moonlighting of cytosolic glyceraldehyde-3-phosphate dehydrogenase regulates Arabidopsis response to heat stress. Nature Communications. (2020-07)
12. Adam W Whisnant, et al. Integrative functional genomics decodes herpes simplex virus 1. Nat Commun. (2020-04-01)
13. Chen J, et al. ACSL4 promotes hepatocellular carcinoma progression via c-Myc stability mediated by ERK/FBW7/c-Myc axis. Oncogenesis. (2020-04)
14. Laia Bonjoch, et al. Germline Mutations in FAF1 Are Associated With Hereditary Colorectal Cancer. Gastroenterology. (2020-03-01)
15. Zhang T, et al. Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis. Cell. (2020-03)
16. Tsai MH, et al. Pathogenic Variants in CEP85L Cause Sporadic and Familial Posterior Predominant Lissencephaly. Neuron. (2020-02)
17. Saskia Hoffmann, et al. Gain-of-function mutations amplify cytotoxic FAM111 protease activity in human genetic disorders. biorxiv. (2020)
18. HaqueMuzammel, et al. The KSHV ORF34 protein Interacts and Stabilizes HIF-2α via Binding to the HIF-2α bHLH and PAS Domains. J. Virol. (2019-06)
19. KimSang-Chul, et al. Interaction and Regulation Between Lipid Mediator Phosphatidic Acid and Circadian Clock Regulators. Plant Cell. (2019-02)
20. ChioUn Seng, et al. A Chaperone Lid Ensures Efficient and Privileged Client Transfer during Tail-Anchored Protein Targeting. Cell Rep. (2019-01)
21. IshiiJun, et al. Congenital goitrous hypothyroidism is caused by dysfunction of the iodide transporter SLC26A7. Commun Biol. (2019-01)
22. Natalia Jiménez-Moreno, et al. LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resilience. biorxiv. (2019)
23. DingLing, et al. PARP1 Suppresses the Transcription of PD-L1 by Poly(ADP-Ribosyl)ating STAT3. Cancer Immunol Res. (2019)
24. Uppal S, et al. The Bromodomain Protein 4 Contributes to the Regulation of Alternative Splicing. Cell Rep. (2019)
25. Park J, et al. UPF1/SMG7-dependent microRNA-mediated gene regulation. Nat Commun. (2019)
26. Zhang J1, et al. Disease-Causing Mutations in SF3B1 Alter Splicing by Disrupting Interaction with SUGP1. Mol Cell. (2019)
27. ZhangJiantao, et al. Identification of NMS-873， an allosteric and specific p97 inhibitor， as a broad antiviral against both influenza A and B viruses. Eur J Pharm Sci. (2019)
28. Paillard Melanie, et al. MICU1 Interacts with the D-Ring of the MCU Pore to Control Its Ca Flux and Sensitivity to Ru360. Mol. Cell. (2018)
29. ZhangYing, et al. Phosphorylation of SMC1A promotes hepatocellular carcinoma cell proliferation and migration. Int. J. Biol. Sci. (2018)
30. Xu Z, et al. UBIAD1 suppresses the proliferation of bladder carcinoma cells by regulating H-Ras intracellular trafficking via interaction with the C-terminal domain of H-Ras. Cell Death Dis. (2018)
31. Rumi MAK,, Singh P,, Roby KF,, Zhao X,, Iqbal K,, Ratri A,, Lei T,, Cui W,,, Borosha S,, Dhakal P,, Kubota K,, Chakraborty D,, Vivian JL,, Wolfe MW,, Soares MJ,,., et al. Defining the Role of Estrogen Receptor β in the Regulation of Female Fertility. Endocrinology. (2017)
32. Davies Stephanie J, et al. Itm2a silencing rescues lamin A mediated inhibition of 3T3-L1 adipocyte differentiation. Adipocyte. (2017)
33. HuJianxin, et al. A G Protein-biased Designer G Protein-coupled Receptor Useful for Studying the Physiological Relevance of Gq/11-dependent Signaling Pathways. J. Biol. Chem. (2016-05)
34. Christensen DP, et al. Reciprocal signals between microglia and neurons regulate α-synuclein secretion by exophagy through a neuronal cJUN-N-terminal kinase-signaling axis. J Neuroinflammation. (2016-03)
35. YingM, et al. The E3 ubiquitin protein ligase MDM2 dictates all-trans retinoic acid-induced osteoblastic differentiation of osteosarcoma cells by modulating the degradation of RARα. Oncogene. (2016)
36. He Y, et al. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature. (2016)
37. Parsons LR, et al. Rapid genome assembly and comparison decode intrastrain variation in human alphaherpesviruses. MBio. (2015)
38. Thompson PJ, et al. hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells. PLoS Genet. (2015)