THE™ DYKDDDDK Tag Antibody, mAb, Mouse
THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK
tagged fusion proteins.
| ￥1000 | |
| A00187-100 | |
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| ￥1000 | |
| A00187-100 | |
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| Specificity |
THE™ DYKDDDDK Tag Antibody, mAb, Mouse recognizes DYKDDDDK tags localized at the C-terminal, N-terminal and internal region of DYKDDDDK
tagged fusion proteins. |
| Host Species | Mouse |
| Immunogen | A synthetic peptide (DYKDDDDK) coupled to KLH |
| Conjugate | Unconjugated |
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Working concentrations for specific applications should be determined by
the investigator. The appropriate concentrations may be affected by secondary
antibody affinity, antigen concentration, the sensitivity of the method of
detection, temperature, the length of the incubations, and other factors. The
suitability of this antibody for applications other than those listed below has
not been determined. The following concentration ranges are recommended
starting points for this product. |
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| Form | Lyophilized |
| Storage Buffer | Lyophilized with PB, pH 6.5, containing 0.02% sodium azide |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent)
to a final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20°C from the
date of receipt. The reconstituted antibody can be stored for 2-3 weeks at
2-8°C.For long term storage, aliquot and store at -20°C or below. Avoid
repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG2b,κ |
| Clonality | Monoclonal |
| Clone ID | 5A8E5 |
| Note |
GenScript can offer this product per customer's request including
product size, buffer components, etc. |
Figure 6. Comparison of DYKDDDDK tag detection of Multiple Tag Protein by THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187; panel A) vs. Mouse Anti- DYKDDDDK Tag mAb (Company S, clone M2; panel B) by Western blot.
In both panels A and B:
Lanes 1-3 400 ng, 80 ng,16 ng Multiple Tag Protein (Purified) (GenScript, M0101)
Lane 4. 10 μL HEK293 cell lysate (negative control)
The primary antibody in panel A was THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) used at 1 μg/mL concentration. The primary antibody in panel B was Mouse Anti- DYKDDDDK Tag mAb (Company S, clone M2) used at 1 μg/mL concentration. In both cases, the secondary antibody was IRDye800 Conjugated Goat Anti-Mouse IgG.
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Figure 2. The affinity of THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) for an N-terminal DYKDDDDK tag was analyzed in an immunocytochemistry/immunofluorescence analysis of N-terminal DYKDDDDK tag protein-transfected HEK293 cells. The primary antibody was THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) used at 1 μg/mL concentration. The secondary antibody was Fluorescein Conjugated Anti-mouse IgG used at 2 μg/mL concentration. »
Figure 5. The detection of DYKDDDDK tag by THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) was analyzed by flow cytometric analysis of non-transfected or DYKDDDDK gene-transfected CHO cells (black and orange, respectively) using THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187). The signal was developed with FITC conjugated Goat Anti-Mouse IgG. »
Figure 7. The affinity of THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) for DYKDDDDK tag in immunoprecipitates from DYKDDDDK-tagged protein-transfected E.coli lysates was analyzed using Western blotting.
Lane 1: E.coli lysate containing DYKDDDDK-tagged fusion protein
Lane 2: Immunoprecipitates of untransformed E.coli lysate incubated with THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 2 µg/mL) and Protein A.
Lane 3: Immunoprecipitates of E.coli lysate containing DYKDDDDK-tagged fusion protein incubated with DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 2 µg/mL) and Protein A.
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Figure 3. Comparison of DYKDDDDK-tag detection by THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187; panel A) vs. Mouse Anti- DYKDDDDK Tag mAb (Company S, clone M2; panel B) by Western blot.
In both panels A and B:
Lane 1 100 ng, 50 ng, 25 ng N-terminal DYKDDDDK-tagged fusion protein (Predicted Size: 52 kDa)
Lane 2 100 ng, 50 ng, 25 ng Internal DYKDDDDK-tagged fusion protein (Predicted Size: 55 kDa)
Lane 3 50 ng, 10 ng, 5 ng C-terminal DYKDDDDK-tagged fusion protein (Predicted Size: 55 kDa)
The primary antibody in panel A was THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) used at 1 μg/mL concentration. The primary antibody in panel B was Mouse Anti- DYKDDDDK Tag mAb (Company S, clone M2) used at 1 μg/mL concentration. In both cases, the secondary antibody was IRDye800 Conjugated Goat Anti-Mouse IgG.
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Figure 1. The affinity of THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) for a C-terminal DYKDDDDK tag was analyzed in an immunocytochemistry/immunofluorescence analysis of C-terminal DYKDDDDK tag protein-transfected HEK293 cells. The primary antibody was THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) used at 1 μg/mL concentration. The secondary antibody was Fluorescein Conjugated Anti-mouse IgG used at 2 μg/mL concentration. »
Figure 4. Consistency analysis of Batch 1# and 2# of THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 1 µg/mL) by Western blot, showing that signal remains consistent from Lot to Lot.
The assay was performed with DYKDDDDK-tagged fusion protein.
The primary antibody were 2 lots of THE™ DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) used at 1 μg/mL concentration. The secondary antibody was IRDye™ 800 Conjugated Goat Anti-Mouse IgG.
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| Target Background |
The DYKDDDDK peptide is a small epitope tag which is widely used in
expression vectors. The tag does not appear to interfere with the bioactivity
or bio-distribution of the tagged recombinant proteins. THE™ DYKDDDDK Tag
Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to
detect DYKDDDDK-tagged proteins |
| Synonyms | Mouse anti DYKDDDDK-tag mAb; Mouse anti flag-tag mAb |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
